论文部分内容阅读
目的本实验采用以CC/MS为基础的代谢物组学为理论,结合现代生物质谱技术,对急性肝衰竭动物模型及其生物人工肝治疗效果进行研究。方法本实验选择杜洛克猪作为肝衰竭动物模型,生物人工肝用肝细胞来自中华实验小型猪。实验分为3组:对照组、生物人工肝组和假手术组。对3组实验动物进行12、18、24、36 h时间点的血样采集,并冻存于-80℃超低温冰箱,直至样品前处理。另外,通过GC/MSr质谱检测对所有样品进行分析,并对所得到的经标准化的数据在数学处理软件MATLAB 7.0进行PCA分析。结果通过PCA分析,对照组因动物发生急性肝衰竭在短期内死亡,而生物人工肝组生存时间显著延长。结论急性肝衰竭猪动物模型具有很好的稳定性,可以反映人体肝衰竭过程的病理过程。
OBJECTIVE: In this study, the theory of metabolomics based on CC / MS was used in combination with modern bio-mass spectrometry to study the therapeutic effect of the animal model of acute liver failure and its bioartificial liver. Methods In this study, Duroc pigs were selected as the animal model of liver failure, and the liver cells of the artificial liver preparations were obtained from the Chinese experimental miniature pigs. The experiment was divided into 3 groups: control group, artificial liver group and sham operation group. Blood samples were taken from the three experimental animals at time points of 12, 18, 24 and 36 h, and stored frozen in -80 ℃ ultra-low temperature refrigerator until sample pretreatment. In addition, all samples were analyzed by GC / MSr mass spectrometry and the resulting normalized data were subjected to PCA analysis in the mathematical processing software MATLAB 7.0. Results By PCA analysis, the control group died of acute liver failure in animals within a short period of time, while the biological artificial liver group significantly prolonged survival time. Conclusion The animal model of acute liver failure has good stability, which can reflect the pathological process of human liver failure.