在微量锆的荧光分析中,已提出很多荧光试剂,使用最普遍的是黄酮类试剂,如桑色素、栎精及其他羟基黄酮。这些荧光反应的灵敏度一般是比较高的。等比较了多种测锆荧光试剂的灵敏度后指出,在大量乙醇的存在下,桑色素和栎精是最灵敏的试剂。我们的实验结果表明,在大量乙醇的存在下,试剂空白的荧光强度是很高的,这势必影响方法的重现性,也限制了灵敏度的进一步提高。 In the fluorescence analysis of trace amounts of zirconium, a number of fluorescent reagents have been proposed, the most commonly used flavonoids, such as morin, quercetin and other hydroxyflavones. The sensitivity of these fluorescent reactions is generally relatively high. After comparing the sensitivity of various zirconium-based fluorescent reagents, it was pointed out that morin and quercetin are the most sensitive reagents in the presence of large amounts of ethanol. Our experimental results show that in the presence of large amounts of ethanol, reagent blank fluorescence intensity is very high, which will inevitably affect the reproducibility of the method, but also limits the further increase of sensitivity.