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目的为实现水中四环素残留的快速检测,探索建立四环素水溶液的表面增强拉曼光谱(surface enhanced Raman spectroscopy,SERS)检测方法。方法以OTR202和OTR103作为表面增强基底,分析四环素水溶液的SERS光谱,应用自适应迭代惩罚最小二乘法(air-PLS)扣除SERS的荧光背景,探讨样品加入量对SERS信号强度的影响,以1274 cm-1作为特征峰建立四环素水溶液的标准曲线。结果确定了四环素水溶液的加入量为20μL,建立的线性回归方程Y=257.47X+85.165,相关系数r为0.9897。结论本研究方法无需前处理,简便快捷,为后续水中四环素残留快速现场检测奠定了良好基础。
Objective To establish a rapid and sensitive method for the determination of tetracycline residues in water, and to establish a surface enhanced Raman spectroscopy (SERS) method for the determination of tetracycline in aqueous solution. Methods OTR202 and OTR103 were used as surface-enhanced substrates to analyze the SERS spectrum of tetracycline solution. The fluorescence background of SERS was deducted by using the adaptive iterative penalty method (air-PLS) to investigate the effect of sample loading on the SERS signal intensity. A 1274 cm -1 as a characteristic peak to establish a standard curve of tetracycline solution. The results showed that the addition of tetracycline solution was 20μL. The linear regression equation was established as Y = 257.47X + 85.165, and the correlation coefficient r was 0.9897. Conclusion The method of this study need not be pre-treatment, which is simple and quick, which lays a good foundation for rapid on-site detection of tetracycline residues in water.