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目的观察3种不同喂养方法对烫伤大鼠免疫功能康复影响,探讨促进烧伤机体免疫功能康复方法。方法健康Wistar大鼠100只,随机分成药膳组、肉汤组、常规组(n=30)和对照组(n=10)。药膳组、肉汤组、常规组于伤后3、71、4 d各取10只,正常对照组假伤后立即处死取材,检测T淋巴细胞亚群和自然杀伤细胞(NK)细胞活性、血浆IgAI、gGI、gM、补体C3、C4含量、肠道sIgA含量。结果烧伤药膳组CD3+、CD4+、NK细胞活性分别为(61.87±6.75)%(、33.14±4.91%)(、9.68±1.31)%,IgAI、gGI、gM、C3、C4及肠道sIgA含量分别为(50.24±8.11)(、197.8±23.2)(、161.9±20.6)(、196.0±24.3)(、73.4±9.4)mg/L和(34.6±8.2)μg/mL,均低于对照组,CD 8+([34.33±2.87)%]高于对照组(P<0.05或P<0.01);与肉汤组、常规组比较,药膳组各免疫指标均恢复较快(P<0.05或P<0.01)。结论药膳喂养可以改善烫伤大鼠T淋巴细胞亚群分布,提高NK细胞活性和肠黏膜细胞sIgA水平,促进机体免疫功能康复。
Objective To observe the effects of three different feeding methods on the immune function of scalded rats, and to explore the methods to promote the immune function of burn rats. Methods One hundred healthy Wistar rats were randomly divided into Diet group, Broth group, conventional group (n = 30) and control group (n = 10). In the diet group, the broth group and the conventional group, 10 rabbits were sacrificed on the 3,71 and 4 d after injury respectively. The rabbits in the normal control group were sacrificed immediately after the injury and the activity of T lymphocyte subsets and natural killer (NK) IgAI, gGI, gM, complement C3, C4 content, intestinal sIgA content. Results The activity of CD3 +, CD4 + and NK cells in burn medicated group were (61.87 ± 6.75)% (, 33.14 ± 4.91%), (9.68 ± 1.31)%, IgAI, gGI, gM, C3, C4 and intestinal sIgA were (50.24 ± 8.11), (197.8 ± 23.2), (161.9 ± 20.6), (196.0 ± 24.3), (73.4 ± 9.4) mg / L and (34.6 ± 8.2) μg / mL, (P <0.05 or P <0.01). Compared with the control group (P <0.05 or P <0.01), the immune indexes in the diet group recovered faster (P <0.05 or P <0.01) . Conclusion Diet can improve the distribution of T lymphocyte subsets in scalded rats, increase the activity of NK cells and the level of sIgA in intestinal mucosa, and promote the recovery of immune function.