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目的:在构建龋病动物模型基础上观察HMME-PDT联合氟制剂预防龋病的效果。方法:选取断奶21d SPF-Wista大鼠80只,种植变形链球菌构建致龋模型后分为蒸馏水、HMME-PDT、氟化钠、以及HMME-PDT+氟化钠组4组,连续8周给大鼠进行口腔处理,采集大鼠唾液培养,记录变形链球菌水平及观察实验组大鼠口内变形链球菌生长繁殖的影响并评估Keyes记分抑制龋损形成的效果。结果:与蒸馏水组相比,其余各组变形链球菌水平和龋齿Keyes记分均有不同程度的降低,其中HMME-PDT显示对窝沟面龋和光滑面龋有较好的防治效果(P<0.05),但均低于氟化钠组;HMME-PDT+氟化钠组未见明显的协同抑龋作用。结论:HMME-PDT能有效抑制口腔变链菌繁殖,降低龋齿的发生率。
OBJECTIVE: To observe the effect of HMME-PDT combined with fluorosis in the prevention of dental caries on the basis of constructing animal model of caries. Methods: Eighty weaned SPF-Wista rats were selected at the age of 21 days. The caries-bearing model was constructed by Streptococcus mutans and then divided into 4 groups: distilled water, HMME-PDT, sodium fluoride and HMME-PDT + The rats were subjected to oral treatment, the saliva cultures of rats were collected, the levels of Streptococcus mutans were recorded and the growth and reproduction of Streptococcus mutans in the mouth were observed and the effect of Keyes score on the inhibition of caries formation was evaluated. Results: Compared with the distilled water group, the levels of Streptococcus mutans and the Keyes score in dental caries decreased to different extents, and the HMME-PDT showed better preventive and therapeutic effects on caries and smooth caries (P <0.05 ), But lower than the sodium fluoride group; HMME-PDT + sodium fluoride group no significant synergistic inhibition of caries. Conclusion: HMME-PDT can effectively inhibit S. mutans reproduction and reduce the incidence of dental caries.