血清视黄醇结合蛋白(Retinol Binding Protein,RBP)是将肝脏中的Vit A 转运到外周组织的载体。目前我国尚不能检测血清RBP,也未见有对血清RBP的分离与纯化的报道。我们以色谱法分离和纯化了人血清RBP。 1 材料和方法 新鲜人全血2000ml来自健康献血者,分离出血清,血清对缓冲液A(20mmol/L磷酸缓冲液,pH7.4)透析过夜,然后离心(10000g,20min), Serum retinin binding protein (Retinol Binding Protein, RBP) is the carrier of the liver in the transport of Vit A to peripheral tissues. At present, our country still can not detect serum RBP, nor has there been any report on the isolation and purification of serum RBP. We isolated and purified human serum RBP by chromatography. MATERIALS AND METHODS Fresh human whole blood 2000 ml was from healthy donors, serum was separated and serum was dialyzed against buffer A (20 mmol / L phosphate buffer, pH 7.4) overnight and then centrifuged (10,000 g, 20 min)