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30多年前,Deinhardt等将1名急性肝炎患者黄疸后第3天的血清接种于狨猴,结果4只接种狨猴全部发生肝炎并可连续传代。进一步实验表明,至少两种因子与感染有关:一种为甲型肝炎病毒(MS-1);另一种命名为GB因子。近年,Mushahwar等证明GB因子与已知的甲、乙、丙、丁、戊型肝炎病毒均不同。将感染GB因子的第11代狨猴血浆接种狷猴,应用减数多聚酶链反应(PCR)方法对狷猴血浆中特异的核苷酸序列
More than 30 years ago, Deinhardt et al. Inoculated the serum of a third day after jaundice in 1 acute hepatitis patient to marmosets. As a result, all four vaccinated marmosets developed hepatitis and were able to pass on continuously. Further experiments showed that at least two factors were related to infection: one was Hepatitis A virus (MS-1); the other was GB factor. In recent years, Mushahwar et al demonstrated that the GB factor is different from the known A, B, C, D and E viruses. The 11th instar monkeys infected with GB factor were inoculated with the plasma of macaque monkeys, and the specific nucleotide sequence in the plasma of marmoset monkey was analyzed by the method of subtractive polymerase chain reaction (PCR)