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目的探讨维吾尔族和汉族弱精子症病人精子mtDNA~(4977bp)缺失的相关性。方法本文采用聚合酶链反应(PCR)技术对60例标本分两组,维、汉各30例,其中精子活力正常标本(对照组)和弱精子症患者(观察组)各15例进行了精子线粒体DNA(mtDNA)缺失的研究。结果发现15例做内对照的维族精子活力正常人中仅有3例(20%)有mtDNA~(4977bp)缺失；而15例维吾尔族弱精子症患者中有10例(67%) mtDNA~(4977bp)缺失；两组4977bp缺失频率比较有显著性差异(P＜0．05)。15例做内对照的汉族精子活力正常标本中无mtDNA~(4977bp)缺失；15例汉族弱精子症患者中有12例(80%)mtDNA~(4977bp)缺失。两组4977bp缺失频率比较差异有显著性(P＜0．05)。同时发现维吾尔族男性不育症与汉族男性不育症mtDNA~(4977bp)缺失频率无显著性差异。结论维吾尔族和汉族mtDNA~(4977bp)缺失在弱精子症的发病中可能起重要作用。
Objective To investigate the correlation between sperm mtDNA ~ (4977bp) deletion in Uygur and Hans asthenospermia patients. Methods In this study, 60 samples were divided into two groups (30 cases in dimension and 30 cases in each) by polymerase chain reaction (PCR) technique. Among them, 15 cases of sperm motility normal samples (control group) and 45 cases of asthenospermia patients (observation group) Study on Mitochondrial DNA (mtDNA) Deletion. The results showed that mtDNA ~ (4977bp) deletion was found in only 3 (20%) of the 15 Uighur sperm motility normal controls. In 15 Uyghur asthenospermia patients, 10 (67%) mtDNA ~ ( 4977bp). There was a significant difference in the frequency of 4977bp deletion between the two groups (P <0.05). There were no mtDNA ~ (4977bp) deletion in 15 normal Chinese sperm motility normal samples, and 12 (80%) mtDNA ~ (4977bp) deletion were found in 15 Han spermatic patients. There was a significant difference in the 4977bp deletion frequency between the two groups (P <0.05). Also found no significant difference in the frequency of mtDNA ~ (4977bp) deletion in male infertility and Han male infertility. Conclusion The deletion of mtDNA ~ (4977 bp) in Uygur and Hans may play an important role in the pathogenesis of asthenospermia.