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脱水应答元件结合蛋白在高等植物应答干旱、高盐和低温胁迫中发挥重要的作用。根据GenBank中小麦(Triticum aestivum L.)DREB基因的cDNA序列设计引物,采用RT-PCR技术从小麦中克隆了DREB基因837 bp的编码区。为进一步研究小麦DREB基因的功能,以pMD18-T-DREB质粒为模板,PCR扩增DREB基因片段,构建了该基因的植物表达载体。经菌液PCR和测序鉴定后,转化到农杆菌LBA4404中,为通过转基因技术深入研究小麦DREB基因的功能奠定了基础。
Dehydro-responsive element-binding proteins play an important role in the response of higher plants to drought, salt and cold stresses. Primers were designed according to the cDNA sequence of DREB gene from wheat (Triticum aestivum L.) in GenBank. The 837 bp coding region of DREB gene was cloned from wheat by RT-PCR. In order to further study the function of wheat DREB gene, the DREB gene fragment was amplified by PCR using pMD18-T-DREB plasmid as a template, and the plant expression vector was constructed. After identification by bacterial PCR and sequencing, it was transformed into Agrobacterium tumefaciens LBA4404, which lays the foundation for the further study on the function of wheat DREB gene by transgenic technology.