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目的拟对当归Angelica sinensis不同部位的化学成分进行比较。方法运用核磁代谢组学技术对当归的不同部位进行了代谢组学化学成分比较。并采用主成分分析、正交偏最小二乘法、单变量分析等统计方法对当归不同部位的代谢产物进行了比较分析。结果通过核磁图谱共指认出包括初级和次级代谢产物共36种。多种统计分析发现,当归头部量较高的成分包括缬氨酸、异亮氨酸等;主要分布于尾部的成分包括Z-丁烯基苯肽、cis-Z,Z′-3α,7α′,7α,3α′-dihydroxyligustilide等;主要分布于身部的成分包括色氨酸、阿魏酸松柏酯;在头部和尾部量均较高的成分包括天冬氨酸、Z-藁本内酯等;在3个部分无明显差异的成分包括丙氨酸、精氨酸等。结论采用的核磁共振指纹可同时表征当归中的初级和次级代谢产物,研究结果可为进一步揭示当归不同部位功效与化学成分的相关性奠定基础。
Objective To compare the chemical composition of different parts of Angelica sinensis Angelica sinensis. Methods Metabolomics chemical composition of different parts of Angelica sinensis were compared by using the method of nuclear magnetic metabonomics. The metabolites of different parts of Angelica sinensis were compared by using principal component analysis, orthogonal partial least squares and univariate analysis. Results A total of 36 primary and secondary metabolites were identified by NMR. A variety of statistical analysis found that the higher the angelica head of the ingredients include valine, isoleucine, etc .; mainly distributed in the tail of the components include Z-butenyl phenyl peptide, cis-Z, Z’-3α, 7α ’, 7α, 3α’-dihydroxyligustilide, etc .; mainly distributed in the body composition, including tryptophan, coniferyl ferulic acid; the amount of the head and tail were higher components include aspartic acid, Z- Esters and the like; components that do not significantly differ in three sections include alanine, arginine and the like. Conclusion The nuclear magnetic resonance fingerprints can characterize the primary and secondary metabolites of Angelica sinensis, and the results may lay the foundation for further revealing the correlation between the efficacy and chemical composition of different parts of Angelica sinensis.