论文部分内容阅读
目的:探讨环氧化酶-1(COX-1)选择性抑制剂莫苯唑酸对牛脂促进的大鼠结肠癌发生的作用.方法:SD♂大鼠共108只.腹腔注射AOM(azoxymethane)后予不同牛脂含量饲料(混合0.12%莫苯唑酸)12wk,通过对结肠总ACF及多隐窝ACF(≥4个异常隐窝/病灶)计数,评估莫苯唑酸对结肠肿瘤形成早期的影响;腹腔注射生理盐水或AOM后予以10%牛脂饲料(混合0.12%莫苯唑酸)44wk,观察莫苯唑酸对肿瘤发病率及数量的影响.BrdU评估肿瘤周围外观正常黏膜的增殖状况,Westernblot检测黏膜β-catenin的表达,探讨莫苯唑酸影响肿瘤发生的可能机制.结果:12wk时,莫苯唑酸组ACF尤其是多隐窝ACF显著低于对照组(P<0.05).44wk时莫苯唑酸组肿瘤发病率及平均肿瘤数量分别为66.7%和2.5±2.3,低于对照组(100%、5.3±1.2)(P<0.05);莫苯唑酸组隐窝增殖细胞的数量均显示出下降趋势(5±0.82vs3.25±0.50;7.75±0.96vs4.75±0.50,均P<0.05),且表现出趋向于生理分布的变化.β-catenin表达均低于对照组(P<0.05).结论:莫苯唑酸可有效抑制牛脂促进的大鼠结肠癌发生,这可能与结肠隐窝上皮细胞β-catenin的表达及增殖受到抑制有关.
OBJECTIVE: To investigate the effect of moxfloxacin, a selective cyclooxygenase-1 (COX-1) inhibitor, on the formation of tallow-induced colon cancer in rats.Methods: A total of 108 SD rats were injected intraperitoneally with azoxymethane The animals were fed with different concentrations of bovine fat (mixed with 0.12% mofetil) for 12 weeks, and the effect of moxfloxacin on the early stage of colon tumor formation was evaluated by counting the total ACF in the colon and ACF (> 4 abnormal crypts / lesions) The effect of moxifloxacin on the incidence and quantity of tumor was observed by intraperitoneal injection of normal saline or AOM for 44 weeks.And BrdU evaluated the proliferation of normal mucosa around the tumor, The expression of β-catenin in mucosa was detected by Western blot and the possible mechanism of the influence of moxfloxacin on tumorigenesis was observed.Results: At 12wk, ACF, especially ACF, in multi-crypts was significantly lower than that in control group (P <0.05) The incidence and the average number of tumors in the moxfloxacin group were 66.7% and 2.5 ± 2.3, respectively, which were lower than those in the control group (100%, 5.3 ± 1.2) (P <0.05) The number showed a downward trend (5 ± 0.82vs3.25 ± 0.50; 7.75 ± 0.96vs4.75 ± 0.50, both P <0.05), and showed a tendency to students (P <0.05) .Conclusion: Moxifloxacin can effectively inhibit the growth of rat colon cancer induced by tallow, which may be related to the β-catenin of colonic crypt epithelial cells Expression and proliferation were inhibited.