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AIM:To study the effect of leflunomide on immunologicaliver injury(ILI)in mice.METHODS:ILI was induced by tail vein injection of 2.5 mgBacillus Calmette-Guerin(BCG),and 10 d later with 10 μglipopolysaccharide(LPS)in 0.2 mL saline(BCG+LPS).Thealanine aminotransferase(ALT),aspartate aminotransferase(AST),nitric oxide(NO)level in plasma and molondiadehyde(MDA),glutathione peroxidase(GSHpx)in liver homogenatewere assayed by spectroscopy.The serum content of tumornecrosis factors-α(TNF-α)was determined by ELISA.Interleukin-1(IL-1),interleukin-2(IL-2)and ConcanavalinA(ConA)-induced splenocyte proliferation response weredetermined by methods of ~3H-infiltrated cell proliferation.RESULTS:Leflunomide(4,12,36 mg·kg~(-1))was found tosignificantly decrease the serum transaminase(ALT,AST)activity and MDA content in liver homogenate,and improvereduced GSHpx level of liver homogenate.Leflunomide(4,12,36 mg·kg~(-1))significantly lowered TNF-α and NO level inserum,and IL-1 produced by intraperitoneal macrophages(PM_Φ).Moreover,the decreased IL-2 production and ConA-induced splenocyte proliferation response were furtherinhibited.CONCLUSION:These findings suggested that leflunomidehad significant protective action on ILI in mice.
AIM: To study the effect of leflunomide on immunological injury (ILI) in mice. METHODS: ILI was induced by tail vein injection of 2.5 mg Bacillus Calmette-Guerin (BCG), and 10 μg later with 10 μg lipopolysaccharide (LPS) (AST), nitric oxide (NO) level in plasma and molondiadehyde (MDA), glutathione peroxidase (GSHpx) in liver homogenate assayed by spectroscopy. The serum content of tumornecrosis factors (TNF-α) was determined by ELISA. Interleukin-1 (IL-1), interleukin-2 (IL-2) and ConcanavalinA (ConA) -induced splenocyte proliferation response were determined by methods of ~ 3H-infiltrated cell proliferation. RESULTS: Leflunomide (4,12,36 mg · kg -1) was found tosignificantly decrease the serum transaminase (ALT, AST) activity and MDA content in liver homogenate, and improvereduced GSHpx level of liver homogenate. Leflunomide (4, 12,36 mg · kg -1) significantly reduced TNF-α and NO level in serum, and IL-1 produced by intraperito neal macrophages (PM_Φ). Moreover, the decreased IL-2 production and ConA-induced splenocyte proliferation response were inhibited further. CONCLUSION: These findings suggest that leflunomide-induced significant protective action on ILI in mice.