目的:通过动物实验观察姜黄水、醇提取液对冰乙酸损伤性溃疡性结肠炎的作用。方法:清洁级大鼠180只,随机分成9组,每组20只:正常对照组、模型组、柳氮磺胺吡啶(SASP)组、姜黄水提液高、中、低剂量组及姜黄醇提液高、中、低剂量组。SASP组给予SASP溶液180 mg/kg灌胃,1次/d;治疗组分别给予姜黄水/醇提液80、40、20 mg/kg,1次/d。所有动物均治疗2 w。第3周断头处死,计算结肠黏膜大体形态损伤分数。采血4 mL,检测血浆丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。取近肛门8 cm处结肠,常规HE染色病理切片并摄像,免疫组化(IHC)检测结肠黏膜肺表面活化蛋白A的表达。结果进行统计学分析。结果:UC模型大鼠的大体形态及组织学损伤评分和血浆MDA含量均较正常大鼠升高,血浆SOD活性下降,结肠黏膜SP-A表达上调。姜黄醇提液高、中剂量组大体形态及组织学损伤评分和MDA含量均显著低于模型组(P<0.01),且与SASP组比较无统计学差异(P>0.05);姜黄醇提取液低剂量组和水提液高剂量组大体形态及组织学损伤评分和血浆MDA含量均显著低于模型组(P<0.01),但与SASP组比较差异显著(P<0.01);水提取液中、低剂量组大体形态及组织学损伤评分和血浆MDA含量与模型组比较无统计学差异(P>0.05)。醇提取液高、中、低组和姜黄水提取物剂量组大鼠结肠黏膜SP-A的表达与模型组比较显著上调(P<0.01),但姜黄醇提取液低剂量组和姜黄水提取物高剂量组上调幅度较小,与SASP组比较差异显著(P<0.01)。水提液中、低济量组SP-A表达水平与模型组比较无显著性差异(P>0.05)。结论:姜黄醇提液能提高机体超氧化物歧化酶活性,降低丙二醛含量,同时上调肺表面活化蛋白A的表达,从而增加结肠黏膜稳定性,减轻脂质过氧化反应损伤,提高机体清除氧自由基的能力,防止溃疡的产生。 OBJECTIVE: To observe the effect of turmeric water and alcohol extract on glacial acetic acid-induced ulcerative colitis in animal experiments. Methods: One hundred and eighty clean-grade rats were randomly divided into nine groups with 20 rats in each group: normal control group, model group, sulfasalazine (SASP) group, high, medium and low dose of turmeric extract and alcohol extract of turmeric Liquid high, medium and low dose group. SASP group was given SASP solution 180 mg / kg orally, once / d; the treatment group were given turmeric water / alcohol extract 80,40,20 mg / kg, 1 time / d. All animals were treated for 2 w. The first three weeks were sacrificed, calculate the general morphology of colonic mucosa damage score. 4 mL of blood was collected for determination of plasma malondialdehyde (MDA) content and superoxide dismutase (SOD) activity. Colon close to the anus at 8 cm was collected and histopathologically stained by routine HE staining. Immunohistochemistry (IHC) was used to detect the expression of pulmonary surfactant protein A in colonic mucosa. Results were statistically analyzed. Results: The morphological and histological damage scores and plasma MDA levels of UC rats were higher than those of normal rats, while the activity of SOD in plasma was decreased and the expression of SP-A in colonic mucosa was up-regulated. The morphology and histological damage scores and the content of MDA of ginger extract in high and medium dose groups were significantly lower than those in model group (P <0.01), and there was no significant difference compared with SASP group (P> 0.05) The morphological and histological damage scores and plasma MDA contents of low-dose group and high-dose water extract group were significantly lower than those of model group (P <0.01), but were significantly different from those of SASP group (P <0.01) There was no significant difference between the low dose group and the model group in gross morphology and histological damage score and plasma MDA level (P> 0.05). The expression of SP-A in colonic mucosa of rats in high, middle and low alcohol extract group and turmeric water extract group was significantly increased compared with model group (P <0.01), but low dose of turmeric alcohol extract group and turmeric water extract The up-regulation of high-dose group was smaller than that of SASP group (P <0.01). In water extract, the expression level of SP-A in low-dose group had no significant difference compared with model group (P> 0.05). CONCLUSION: The turmeric alcohol extract can increase the activity of superoxide dismutase, reduce the content of malondialdehyde and up-regulate the expression of pulmonary surfactant protein A, thereby increasing the stability of colonic mucosa, reducing the damage of lipid peroxidation and improving the clearance of the body The ability of oxygen free radicals to prevent ulcer production.