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目的:比较辅助生殖技术中快冻(玻璃化法)和慢冻(程序化法)两种冷冻方法对卵裂期胚胎的冻融效果。方法:将复苏后全部卵裂球存活的胚胎定义完整存活胚胎,同一病人由于有多余冻融胚胎而行2次及以上移植周期的称为重复周期。对577个快冻周期和276个慢冻周期的相关资料进行回顾性统计学分析,比较两组冻融胚胎的存活率、完整存活率、重复周期率、胚胎种植率和临床妊娠率等指标。结果:快冻组的每周期胚胎解冻数低于慢冻组,而每周期存活胚胎数和重复周期率均高于慢冻组;玻璃化冷冻后的胚胎存活率和完整性胚胎存活率均显著高于慢冻组,复苏周期移植取消率显著低于慢冻组,而胚胎种植率和临床妊娠率则明显高于慢冻组,均有统计学差异(P<0.05)。结论:玻璃化法能够较好地保存冻融胚胎的发育潜能,得到较好的临床妊娠率,是一种卵裂期胚胎冷冻保存的理想方法。
OBJECTIVE: To compare the effects of both freezing (vitrification) and slow freezing (programmed) methods on the freezing and thawing of cleavage stage embryos in assisted reproductive technology. Methods: All surviving embryos after resuscitation were defined as surviving embryos. Two or more cycles of the same patient with redundant frozen-thawed embryos were referred to as repetitive cycles. Retrospective statistical analysis was performed on 577 fast-freeze cycles and 276 slow-freeze cycles. The survival rate, complete survival rate, repetition cycle rate, embryo implantation rate and clinical pregnancy rate of the two groups of frozen-thawed embryos were compared. Results: The number of embryos thawed in the fast-frozen group was lower than that of the slow-frozen group, while the number of surviving embryos per cycle and the repetitive cycle rate were higher in the frozen-thawed group than those in the slow-frozen group. The survival rate of vitrified embryos and the complete embryo survival rate were significantly The rate of ablation in the resuscitation cycle was significantly lower than that of the slow-frozen group, but the embryo implantation rate and clinical pregnancy rate were significantly higher than those in the slow-frozen group (P <0.05). Conclusion: The vitrification method can well preserve the developmental potential of frozen-thawed embryos and obtain a good clinical pregnancy rate. It is an ideal method for cryopreservation of cleavage stage embryos.