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Objective: To observe the effects of immunosuppressants triptolide (TL) andcyclosporine A (CSA) on HLA antigens expression induced by interferon-γ(INF -γ) invitro.Method; By using an indirect immunofluorescent method and analysing withACAS-570, the abnormal HLA antigen expression of cultured corneal epithelial cellswas induced by INF-r. After incubation with one of the immunosuppressants (CSA, TL)for 72 hrs, the amount of HLA-A BC and HLA-DR antigens was measured.Result: There was no significant difference ( P > 0. 05) between the group with CSAand the positive control group without CSA. In contrast to CSA, TL dramaticallyinhibited INF-γ induced expression of HLA antigens of corneal epithelial cells ( P <0. 001), compared with the control group without TL.Conclusion: TL had direct inhibition on the expression of HLA-ABCand HLA-DRantigens induced by INF-γ in vitro, while CSA had no obvious inhibition. Eye Science2000; 16: 34 - 37.
Objective: To observe the effects of immunosuppressants triptolide (TL) andcyclosporine A (CSA) on HLA antigens expression induced by interferon-γ (INF-γ) invitro.Method; By using an indirect immunofluorescent method and analysing with ACAS-570, the abnormal HLA antigen expression of cultured corneal epithelial cells was induced by INF-r. After incubation with one of the immunosuppressants (CSA, TL) for 72 hrs, the amount of HLA-A BC and HLA-DR antigens was measured. Result: There was no significant In contrast to CSA, TL-drafted INF-γ induced expression of HLA antigens of corneal epithelial cells (P <0.001), compared with the difference (P> 0.05) between the group with CSA and the positive control group without CSA. control group without TL.Conclusion: TL had direct inhibition on the expression of HLA-ABC and HLA-DRantigens induced by INF-γ in vitro, while CSA had no obvious inhibition. Eye Science 2000; 16: 34-37.