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目的探讨IgA肾病患者外周血B淋巴细胞β1,3半乳糖基转移酶(β1,3GT)mRNA和其分子伴侣(Cosmc)mRNA表达水平与IgA肾病临床及病理的关系。方法 2006年5月-2007年2月在中山大学附属第一医院肾活检确诊为IgA肾病的23例患儿(IgA肾病组),按照病理分级分为Ⅰ级+Ⅱ级组(n=6)、Ⅲ级组(n=6)和Ⅳ级+Ⅴ级组(n=11)。同期本院非IgA肾病患儿10例为非IgA肾病组,本院体检健康儿童23例为健康对照组。3组儿童年龄、性别比较差异均无统计学意义。分离3组儿童外周血B淋巴细胞,提取RNA,应用实时荧光定量反转录-PCR检测外周血B淋巴细胞β1,3GT mRNA、Cosmc mRNA表达水平。结果 IgA肾病患儿外周血B淋巴细胞β1,3GTmRNA表达水平(0.88±0.17)与健康对照组(0.79±0.20)和非IgA肾病组儿童(0.84±0.21)比较差异均无统计学意义(P>0.05)。IgA肾病患儿外周血B淋巴细胞Cosmc mRNA表达水平(0.82±0.20)低于健康对照组(0.99±0.32)和非IgA肾病(1.03±0.25)(P<0.05)。IgA肾病患儿24h尿蛋白水平与外周血B淋巴细胞β1,3GT mRNA、Cosmc mRNA表达水平均呈负相关(Pa<0.05)。Ⅰ级+Ⅱ级组、Ⅲ级组、Ⅳ级+Ⅴ级组IgA肾病患儿外周血B淋巴细胞β1,3GT mRNA、Cosmc mRNA表达水平无统计学差异(Pa>0.05)。结论 IgA肾病患者外周血B淋巴细胞Cosmc mRNA表达水平降低,与临床表现密切相关。外周血B淋巴细胞Cosmc mRNA表达水平降低可能与IgA肾病的发病有关。
Objective To investigate the relationship between the expression of β1, 3-galactosyltransferase (β1, 3GT) mRNA and the expression of Cosmc mRNA in IgA nephropathy patients and the clinical and pathological features of IgA nephropathy. Methods Twenty-three children with IgA nephropathy (IgA nephropathy) diagnosed by renal biopsy in the First Affiliated Hospital of Sun Yat-sen University from May 2006 to February 2007 were divided into grade Ⅰ and grade Ⅱ (n = 6) , Grade Ⅲ (n = 6) and grade Ⅳ + Ⅴ (n = 11). In the same period, 10 non-IgA nephropathy children in our hospital were non-IgA nephropathy group, and 23 healthy children in our hospital were healthy control group. There was no significant difference between the three groups in age and sex. The peripheral blood B lymphocytes were isolated from 3 groups of children and RNA was extracted. The expression of β1, 3GT mRNA and Cosmc mRNA of peripheral blood B lymphocytes were detected by real-time fluorescence quantitative reverse transcription-PCR. Results The levels of β1, 3GT mRNA in peripheral blood of IgA nephropathy patients (0.88 ± 0.17) were not significantly different from those in healthy controls (0.79 ± 0.20) and non-IgA nephropathy patients (0.84 ± 0.21) (P> 0.05). The expression level of Cosmc mRNA in B lymphocytes of children with IgA nephropathy (0.82 ± 0.20) was lower than that of healthy controls (0.99 ± 0.32) and non IgA nephropathy (1.03 ± 0.25) (P <0.05). The levels of urinary protein in 24h with IgA nephropathy were negatively correlated with the expression of β1, 3GT mRNA and Cosmc mRNA (P <0.05). The levels of β1, 3GT mRNA and Cosmc mRNA in B lymphocytes in grade Ⅰ + Ⅱ group, grade Ⅲ group and grade Ⅳ + Ⅴ group were not statistically different (P> 0.05). Conclusion The expression of Cosmc mRNA in B lymphocytes from patients with IgA nephropathy is decreased, which is closely related to clinical manifestations. The decrease of Cosmc mRNA expression in peripheral blood B lymphocytes may be related to the pathogenesis of IgA nephropathy.