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目的探讨DNA修复能力(DRC)与肝细胞癌(HCC)的关联性。方法运用彗星试验,检测150例新发HCC患者(病例组)和150例非肿瘤患者(对照组)的DNA修复能力。结果博莱霉素(BLM)暴露前,病例组彗星尾长和尾矩分别为(3.98±2.35)和(1.41±0.85),对照组尾长和尾矩分别为(3.60±2.04)和(1.24±0.66),2组尾长和尾矩差异均无统计学意义(P>0.05);BLM暴露后经15min修复,病例组尾长和尾矩分别为(6.60±3.11)和(2.59±1.56),对照组尾长和尾矩分别为(4.69±2.44)和(1.58±0.96),2组尾长和尾矩差异均有统计学意义(P<0.01);用尾长作为检测指标计算DRC时,2组DRC中位数分别为78.65%和93.81%,差异有统计学意义(P<0.01);用尾矩作为检测指标计算DRC时,2组DRC中位数分别为77.78%和93.11%,差异有统计学意义(P<0.01)。结论 DNA修复能力低下可能是HCC发生的高危因素之一,可作为预警人群中HCC发病的生物标志物。
Objective To investigate the association between DNA repair capacity (DRC) and hepatocellular carcinoma (HCC). Methods Comet assay was used to detect DNA repair capacity in 150 newly diagnosed HCC patients (case group) and 150 non-tumor patients (control group). Results Before bleomycin (BLM) exposure, the tail length and tail moment of the comet were (3.98 ± 2.35) and (1.41 ± 0.85), respectively. The tail length and tail moment of the control group were (3.60 ± 2.04) and ± 0.66). There was no significant difference in tail length and tail moment between the two groups (P> 0.05). After BLM exposure for 15 minutes, the tail length and tail moment of the group were (6.60 ± 3.11) and (2.59 ± 1.56) , While the tail length and tail moment in the control group were (4.69 ± 2.44) and (1.58 ± 0.96), respectively. There were significant differences in the tail length and tail moment between the two groups (P <0.01) . The median DRC scores of two groups were 78.65% and 93.81%, respectively, with significant difference (P <0.01). Median DRC of two groups were 77.78% and 93.11% The difference was statistically significant (P <0.01). Conclusion The low DNA repair capacity may be one of the risk factors of HCC, which can be used as a biomarker of HCC in early warning population.