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气体信号分子硫化氢(H2S)通过对蛋白质巯基化修饰参与动物体内许多重要的生理活动,但是在植物中该方面的报道甚少.本论文证明,H2S信号提高蛋白质的巯基化水平参与谷子(foxtail millet)对低温、高温、盐、渗透和紫外胁迫的响应.用5种方式4℃、42℃、Na Cl、PEG和UV处理,引起谷子H2S产生速率和内源H2S含量的升高.实时荧光定量PCR检测上述5种处理后谷子幼苗H2S产生酶基因Lcd1、Lcd2、Dcd1、Dcd2和Des的表达,Lcd1受盐、低温和渗透胁迫诱导;Lcd2受高温、低温和渗透胁迫诱导;Dcd1受盐诱导;Dcd2和Des受低温诱导;UV处理后5个酶基因表达均下降.Western印迹检测表明,外源H2S熏蒸和5种胁迫处理引起谷子幼苗蛋白质巯基化水平升高.上述结果证明,H2S信号通过提高蛋白质的巯基化水平参与谷子对低温、高温、盐、渗透和紫外胁迫的响应.
Gas signaling molecule Hydrogen sulfide (H2S) is involved in many important physiological activities in animals through thiolation of proteins, but few reports have been reported in plants.This paper demonstrates that H2S signaling enhances the level of thiolation of proteins involved in foxtail millet response to low temperature, high temperature, salt, osmotic stress and UV stress.The results showed that H2S production rate and endogenous H2S content in millet increased with 5 treatments of 4 ℃, 42 ℃, NaCl, PEG and UV.Fluorescence The expression of Lcd1, Lcd2, Dcd1, Dcd2 and Des was detected by quantitative PCR. The expression of Lcd1 was induced by salt, low temperature and osmotic stress. Lcd2 was induced by high temperature, low temperature and osmotic stress. Dcd1 was induced by salt ; Dcd2 and Des were induced by low temperature and the expression of 5 enzymes decreased after UV treatment.Western blotting showed that the H2S signaling was enhanced by exogenous H2S fumigation and 5 stress treatments Increasing the thiolation level of proteins is involved in the response of millet to low temperature, high temperature, salt, osmotic and UV stress.