论文首次报道了利用49种微生物菌株对人参皂苷Rg1进行生物转化研究,发现小型丝状真菌黑曲霉(Aspergillus niger 3.1858)与蓝色梨头霉(Absidia coerulea 3.3538)具有转化能力。经正交试验优化转化条件,放大培养,制备得到产物MT1与MT2,经化学与波谱技术鉴定,MT1与MT2为同一产物即人参皂苷Rh1。运用TLCS方法探索了真菌体系中底物人参皂苷Rg1与转化产物人参皂苷Rh1的生物转化动力学曲线。 For the first time, the paper reported the biotransformation of ginsenoside Rg1 using 49 strains of microorganisms. It was found that the small filamentous fungi Aspergillus niger 3.1858 and Absidia coerulea 3.3538 had transformation ability. The orthogonal test was used to optimize the transformation conditions, and the culture was expanded to obtain the products MT1 and MT2. Chemically and spectroscopically, MT1 and MT2 were identical products, namely ginsenoside Rh1. The biotransformation kinetic curve of the substrate ginsenoside Rg1 and the transformed product ginsenoside Rh1 in the fungal system was explored by TLCS method.