Objective:To explore the effect of wujiadan Recipe on the expression of FSH, E2 in serum and KiSS-1, GPR54, GnRH mRNA in hypothalamic arcuate nucleus tissue homogenate of ovariectomized rats, and to further explore the mechanism of wujiadan Recipe on menopausal syndrome.Methods:Sixty-four adult female rats were randomly divided into three groups: blank group, model group, Chinese medicine control group, west medicine control group, and wujiadan high, medium and low dose groups. Except for the blank group, all the other groups were ovariectomized. The rats in the normal group and the model group were given the same dose of saline once a day. One rat in the blank group died during the experiment. After 4 weeks of continuous administration, blood was collected from abdominal aorta and brain stereotaxic localization was performed to preserve the arcuate nucleus of hypothalamus. The levels of FSH and E2 in serum were detected by enzyme-linked immunosorbent assay (ELISA), and the expression of Kiss-1, GPR54 and GnRH mRNA in arcuate nucleus of hypothalamus were detected by PCR.Results:1. Compared with the blank group, FSH level in the model group was significantly higher (P < 0.01), and E2 level was significantly lower (P < 0.01). Compared with the model group, the level of serum E2 in the west medicine control group increased significantly (P. The serum FSH content in each group decreased, especially in the high dose group and the west medicine control group (P < 0.01), and in the Chinese medicine control group, the middle dose group and the low dose group (P < 0.05). Compared with the blank group, the expression of KiSS-1 mRNA, GPR54 mRNA and GnRH mRNA in the model group increased significantly (P < 0.01); compared with the model group, the expression of KiSS-1 mRNA and GPR54 mRNA in the high dose group, middle dose group and West medicine control group of wujiadan prescription decreased significantly (P < 0.05). Compared with the model group, the expression of GnRH mRNA in the high dose group and the west medicine control group decreased significantly (P < 0.01); the middle dose group of wujiadan decreased significantly (P < 0.05).Conclusion: Wujiadan recipe can improve the serum sex hormone level of castrated rats, and reduce the over expression of KiSS-1, GPR54 and GnRH mRNA in hypothalamic arcuate nucleus of castrated rats, which may be the reproductive endocrine mechanism of wujiadan recipe in the treatment of menopausal syndrome.