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目的通过检测增殖蛋白Ki67在正常妊娠各期和FGR患者胎盘组织中的表达,探讨其在正常妊娠中的作用及与FGR的关系。方法采取免疫组化法取正常妊娠各期和FGR患者胎盘组织共42例,检测Ki67蛋白的表达。结果(1)Ki67主要在细胞滋养细胞中表达强,在合体滋养细胞中呈阴性。(2)Ki67在早、中、晚期胎盘组织阳性表达率无统计意义(P>0.05),表达强度早、中期及中、晚期无显著差别(P>0.05);早、晚期有显著差异(P<0.05);FGR组与正常妊娠晚期组胎盘中Ki67表达阳性率、表达强度之间无显著差异(P>0.05)。结论(1)Ki67在妊娠早期、中期表达强度高,说明滋养细胞处于高增殖状态。(2)Ki67随妊娠进展表达下调,提示与胎盘老化和分娩发动有关。(3)FGR组Ki67表达阳性率上调说明细胞滋养细胞的增生。
Objective To detect the expression of proliferating cell nuclear antigen (Ki67) in normal pregnancy and placenta of FGR patients, and to explore its role in normal pregnancy and its relationship with FGR. Methods Totally 42 cases of normal pregnancy and placental tissue from patients with FGR were taken immunohistochemically to detect the expression of Ki67 protein. Results (1) Ki67 is mainly expressed strongly in cytotrophoblasts and negative in syncytiotrophoblasts. (2) There was no significant difference of Ki67 expression in early, middle and late stages of placenta (P> 0.05). There was no significant difference in early, middle, middle and late stages of Ki67 expression (P> 0.05) <0.05). There was no significant difference in the expression of Ki67 between the FGR group and the normal pregnancy group (P> 0.05). Conclusion (1) Ki67 expression in early pregnancy, mid-term high intensity, indicating that trophoblast cells in a high proliferative state. (2) Ki67 downregulation with the progress of pregnancy, suggesting that the aging and delivery of the placenta related. (3) The up-regulation of Ki67 expression in FGR group shows the proliferation of cytotrophoblast.