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分析中国实验用小型猪肝细胞型生物人工肝支持系统 (BALSS)替代衰竭肝功能的可能机制。使少肝急性肝衰 (ALF)犬血液或 16 40培养液循环流经BALSS中空纤维管的内腔 ,与培养于中空纤维管外腔中的猪肝细胞营养液进行物质交换。测定 16 40液中利多卡因浓度、白蛋白和尿素浓度的变化 ;用蛋白电泳法测定 16 40液和猪肝细胞营养液中蛋白成分的变化 ;用透射电镜观察治疗ALF犬时BALSS中猪肝细胞超微结构的变化。结果显示BALSS循环 6h时 ,16 40液中利多卡因浓度明显降低 ;白蛋白和尿素浓度明显升高 ;分子量小于 10 0kD的蛋白质成分明显增多。BALSS治疗ALF犬 6h时 ,猪肝细胞的超微结构出现明显的损伤。提示此型BALSS的猪肝细胞通过透析膜可较好地发挥肝细胞的生物转化及合成代谢等功能 ,以此达到暂时替代衰竭肝脏功能的效应。
To analyze the possible mechanism of experimental small pig hepatocyte-type bioartificial liver support system (BALSS) in replacing failed liver function in China. Acute liver failure (ALF) canine blood or 16 40 medium was circulated through the lumen of the hollow fiber tube of BALSS for material exchange with the nutrient solution of porcine hepatocyte cultured in the external cavity of the hollow fiber tube. The changes of lidocaine concentration, albumin and urea concentration in 1640 liquid were measured. The protein components of 1640 liquid and porcine hepatocyte nutrient solution were determined by protein electrophoresis. Cell ultrastructure changes. The results showed that when the BALSS circulation was 6h, the concentration of lidocaine in 1640 liquid was significantly decreased; the albumin and urea concentrations were significantly increased; and the protein components with the molecular weight less than 100kD were obviously increased. At 6 hours after ALT treatment, the ultrastructure of pig hepatocytes showed obvious damage. Tip of this type of BALSS pig liver cells through the dialysis membrane can better play the biological transformation of liver cells and anabolic and other functions, so as to temporarily replace the effect of failure of liver function.