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目的观察腹腔注射环磷酰胺对小鼠精子胞内钙离子浓度的影响。方法将20只雄性昆明小鼠随机分为对照组和模型组,按60mg/kg体重给对照组小鼠腹腔注射生理盐水(NS),另按60mg/kg体重给模型组小鼠腹腔注射环磷酰胺,每天1次,连续5d,常规饲养观察34d,然后对小鼠精子进行精液常规分析,对精子行FITC-AV/PI染色后用流式细胞仪检测精子凋亡率,用Ca~(2+)荧光探针Fluo-3/AM处理精子后用流式细胞仪检测精子胞内Ca~(2+)浓度。结果对照组与模型组精子密度分别为(5.20±1.34)×10~6/ml和(1.73±0.03)×10~6/ml,精子活力分别为(14.49±0.30)%和(6.64±1.88)%,精子活率分别为(68.39±1 5.1 3)%和(39.96±4.89)%,精子凋亡率(AV/PI)分别为(8.38±0.45)和(14.84±1.22),胞内Ca~(2+)浓度分别为(133.14±11.86)和(80.84±6.92),以上项目两组比较差异均有统计学意义(P<0.05)。结论腹腔注射环磷酰胺可使小鼠精子密度、活力、活率降低,精子凋亡增加,精子胞内Ca~(2+)浓度降低。造成精子胞内Ca~(2+)浓度降低的可能机理是环磷酰胺降低了精子特异性Ca~(2+)通道蛋白功能。
Objective To observe the effect of intraperitoneal injection of cyclophosphamide on the intracellular calcium concentration of sperm in mice. Methods Twenty male Kunming mice were randomly divided into control group and model group. The control group was intraperitoneally injected NS with 60mg / kg body weight and the model group was intraperitoneally injected with cyclophosphamide Spermine for 5 days. The sperm were routinely cultured for 34 days. The spermatozoa were sperm routinely analyzed. The apoptosis rate of sperm was detected by FITC-AV / PI staining. +) Fluorescence probe Fluo-3 / AM after treatment of sperm by flow cytometry sperm intracellular Ca ~ (2+) concentration. Results The sperm motility of control group and model group were (5.20 ± 1.34) × 10 ~ 6 / ml and (1.73 ± 0.03) × 10 ~ 6 / ml respectively and the sperm motility were (14.49 ± 0.30)% and (6.64 ± 1.88) % And sperm motility were (68.39 ± 1 5.13)% and (39.96 ± 4.89)% respectively, and the sperm apoptosis rate (AV / PI) were 8.38 ± 0.45 and 14.84 ± 1.22 respectively. (2+) concentrations were (133.14 ± 11.86) and (80.84 ± 6.92) respectively. There were significant differences between the two groups in the above items (P <0.05). Conclusion Intraperitoneal injection of cyclophosphamide can reduce the density, viability and viability of sperm, increase sperm apoptosis and decrease intracellular Ca 2+ concentration in sperm. The possible mechanism of the decrease of intracellular Ca2 + concentration in sperm is that cyclophosphamide reduces sperm-specific Ca2 + channel protein function.