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目的 :探讨乙型肝炎病毒 (HBV)对肾脏的致病作用。方法 :应用地高辛素标记的HBVDNA两种探针原位杂交 (ISH) ,检测 15例乙型肝炎病毒相关性肾炎 (HBV -GN)患者肾活检石腊包埋切片HBVDNA。结果 :ISH采用HBVDNA全长段探针 9/15例阳性 (6 0 % ) ,其中 5例阳性和 4例阴性者再用HBVDNAX +C段探针检查 ,各获 1例阳性 (2 0 %和 2 5 % ) ,阳性信号主要见于肾小管上皮细胞 ,其次是肾小球系膜细胞 ,呈胞浆型或核型。结论 :用ISH技术检测证实HBV -GN患者肾组织中存在HBVDNA ,提示HBV可能对肾脏有直接致病作用。HBV在肾脏复制现象有待进一步研究证实
Objective: To investigate the pathogenic effect of hepatitis B virus (HBV) on the kidney. Methods: HBVDNA was detected by in situ hybridization (ISH) with digoxigenin-labeled HBVDNA in 15 patients with hepatitis B virus-associated nephritis (HBV-GN). Results: HBsAg full-length probes were positive in 9/15 cases (60%) in ISH, of which 5 were positive and 4 were negative, followed by HBVDNAX + C probe, one case was positive (20% and 25%). The positive signals were mainly found in renal tubular epithelial cells, followed by mesangial cells, showing cytoplasmic or karyotype. Conclusion: HBsAg is detected in renal tissue of patients with HBV-GN by ISH technique, suggesting that HBV may have a direct pathogenic effect on the kidney. HBV replication in the kidney needs further study confirmed