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为了解决野生藏药资源日益濒危问题,推动藏药野生资源保护与适宜区的发展和提供人工栽培种植新方法,该文采用HPLC对不同产地中国沙棘叶中槲皮素、山柰素、异鼠李素进行含量测定,应用偏最小二乘回归法(PLSR),分析不同产地中国沙棘叶中黄酮类化学成分与生态因子间的相关性。该文基于Maxent模型结合运用ArcGIS软件,对中国沙棘进行适宜区区划研究。分析得出不同产地间中国沙棘叶中槲皮素、山柰素、异鼠李素含量差异明显,且影响槲皮素积累的主导因子为海拔高度、1月份均降水量和8月份均降水量,影响山柰素含量积累的主导因子为海拔高度、最冷季度降水量、12月份均降水量和3月份均气温,影响异鼠李素含量积累的主导因子为8月份均降水量、1月份均降水量、最冷季度降水量和海拔高度;实验结果表明中国沙棘在我国地区分布的适宜指数为0~0.708,适宜区面积59.05万km~2,占全国总面积的6.13%,较适宜区面积达55.25万km~2,占全国总面积的5.73%。对中国沙棘叶黄酮类成分与生态因子的相关性及中国沙棘生态适宜性研究,该法操作简单可行、结果可靠,为藏药资源实现可持续开发利用提供了一种新思路。
In order to solve the problem of endangered wild Tibetan medicinal resources, promote the development of wild Tibetan medicinal resources and the suitable area, and provide a new cultivation method for artificial cultivation, this paper uses HPLC to study the effects of quercetin, kaempferol, The contents of flavonoids in leaves of Chinese seabuckthorn from different habitats were analyzed by partial least squares regression (PLSR), and their correlations were analyzed. Based on the Maxent model combined with ArcGIS software, this paper studies the suitable regional division of Chinese seabuckthorn. The results showed that the contents of quercetin, kaempferol and isorhamnetin in Chinese seabuckthorn leaves were significantly different among different producing areas. The dominant factors affecting the accumulation of quercetin were the altitude, the average precipitation in January and the average precipitation in August The dominant factors affecting the accumulation of beryl chalcogenide content are the altitude, the coldest quarterly precipitation, the average precipitation in December and the average temperature in March. The dominant factor affecting the accumulation of isorhamnetin is August precipitation, and January Average precipitation, the coldest precipitation and altitude. The results showed that the suitable index of Chinese seabuckthorn distribution in China was 0 ~ 0.708, the suitable area was 590,500 km ~ 2, accounting for 6.13% of the total area of China, the suitable area An area of 552,500 km2, accounting for 5.73% of the total area. The correlation between flavonoids in Chinese seabuckthorn leaves and ecological factors and the ecological suitability of Chinese seabuckthorn were studied. The method was simple, feasible and reliable, which provided a new idea for the sustainable development and utilization of Tibetan medicinal resources.