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目的建立穿山龙的高效液相色谱指纹图谱,研究其化学成分与抑制肿瘤细胞增殖活性的相关性,为该药的质量控制提供依据。方法色谱柱为Diamonsil C18柱(250 mm×4.6 mm,5μm),流动相为水和乙腈,采用梯度洗脱方式,柱温为35℃,检测波长为210 nm。对样品进行高效液相色谱指纹图谱分析和抑制肿瘤细胞的增殖活性试验,采用相关分析和多元线性回归分析方法将活性数据与各指纹峰数据相关联。结果在HPLC指纹图谱中,确立了20个共有峰,其中10个色谱峰与抗癌活性显著相关,这10个化学成分可能是穿山龙抗肿瘤的活性成分;结合多元线性回归分析结果与系统聚类分析结果,可将样品按照活性高低分类。结论穿山龙高效液相色谱指纹图谱结合其抗癌活性研究可为评价穿山龙药材的质量提供依据。
OBJECTIVE: To establish a HPLC fingerprint of Chimonanthus praecox, to study the correlation between its chemical composition and the activity of inhibiting the proliferation of tumor cells, and to provide a basis for the quality control of this drug. Methods The column was Diamonsil C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of water and acetonitrile. The gradient elution method was used. The column temperature was 35 ℃ and the detection wavelength was 210 nm. The samples were analyzed by high performance liquid chromatography (HPLC) and inhibition of tumor cell proliferative activity. Correlation analysis and multiple linear regression analysis were used to correlate the activity data with each fingerprint peak data. Results In the HPLC fingerprinting, 20 common peaks were established. Among them, 10 peaks were significantly correlated with the anticancer activities. These 10 chemical components may be the antitumor active ingredients of S. aureus. Combining the results of multivariate linear regression with those of system clustering The results of the analysis, the samples can be classified according to the level of activity. Conclusion The HPLC fingerprint of S. japonicus combined with its anti-cancer activity may provide a basis for the evaluation of the quality of the medicinal herbs.