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目的探讨微卫星杂合性缺失(LOH)在复发性喉鳞状细胞癌患者原发癌的特征及其临床意义。方法选取染色体3p、5p和9p上3个多态性微卫星位点D3S1234、D5S592、D9S171,利用PCR-SSCP银染技术,对已复发的31例原发喉鳞状细胞癌组织及相应癌旁组织进行LOH分析。结果 3个微卫星标记物LOH发生率分别为48.3%(15/31),45.2%(14/31),61.3%(19/31)。LOH的检出率与第一次术后至复发时间(复发时间)呈负相关,即检出患者术后复发时间短,未检出患者术后复发时间长,两组间差异有统计学意义(P<0.05)。结论微卫星杂合性缺失与术后复发时间呈负相关,可作为喉癌预测术后复发的参考标志,有效指导术后临床治疗方案的选择。
Objective To investigate the characteristics and clinical significance of microsatellite heterozygosity deletion (LOH) in primary carcinoma of recurrent laryngeal squamous cell carcinoma. Methods Three polymorphic microsatellite loci, D3S1234, D5S592 and D9S171, on chromosomes 3p, 5p and 9p were selected and 31 cases of primary laryngeal squamous cell carcinoma and corresponding adjacent Organize LOH analysis. Results The incidence of three microsatellite markers LOH were 48.3% (15/31), 45.2% (14/31) and 61.3% (19/31), respectively. The detection rate of LOH was negatively correlated with the time from the first postoperative recurrence (recurrence time), that is, the time of postoperative recurrence was short, the time of postoperative recurrence was not detected, and the difference between the two groups was statistically significant (P <0.05). Conclusion The loss of heterozygosity of microsatellites is negatively correlated with the time of postoperative recurrence, which can be used as a reference mark for predicting the recurrence of laryngeal cancer, effectively guiding the choice of clinical treatment options.