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目的探讨清肝滋肾中药治疗原发性高血压病合并代谢紊乱的可能作用机制。方法 18只自发性高血压大鼠随机分为正常组、模型组和中药组,每组6只。正常组在普通饲料喂养基础上予0.5%纤维素钠安慰剂1 ml灌胃,模型组在高脂饲料喂养基础上予0.5%纤维素钠安慰剂1 ml灌胃,中药组在高脂饲料喂养基础上给予清肝滋肾中药22.5 g/(kg·d)混悬液灌胃,各组均灌胃8周。灌胃4周及8周时,测定血清中血管紧张素Ⅱ(AngⅡ)、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、单核细胞趋化蛋白1(MCP-1)、人纤溶酶原激活物抑制1(PAI-1)水平。结果高脂喂养后即8周时模型组大鼠血清AngⅡ、TNF-α、IL-6、PAI-1水平较正常组均显著升高。而给予清肝滋肾中药干预后,与模型组比较,中药组大鼠血清AngⅡ、TNF-α、IL-6、PAI-1水平显著降低,其中IL-6与正常组差异无统计学意义(P>0.05),TNF-α显著低于正常组,PAI-1显著高于正常组(P<0.05)。结论清肝滋肾中药可以抑制高脂喂养自发性高血压大鼠炎症因子的表达,可能是其治疗高血压病合并代谢紊乱作用机制之一。
Objective To explore the possible mechanism of “Qinggan Zishen Chinese medicine” in treating essential hypertension complicated with metabolic disorder. Methods 18 spontaneously hypertensive rats were randomly divided into normal group, model group and TCM group, with 6 rats in each group. The normal group was fed with 0.5% sodium cellulose placebo 1 ml intragastrically on the basis of normal feed. The model group was given intragastrically with 0.5% sodium cellulose placebo 1 ml on the basis of high fat diet. The rats in the traditional Chinese medicine group were fed with high fat diet On the basis of given Ganganzizhen Chinese medicine 22.5 g / (kg · d) suspension gavage, each group were gavage for 8 weeks. Serum levels of Ang Ⅱ, TNF-α, IL-6, and MCP-1 were measured at 4 and 8 weeks after gavage. 1), human plasminogen activator inhibitor 1 (PAI-1) levels. Results Serum levels of AngⅡ, TNF-α, IL-6 and PAI-1 in model group were significantly higher than those in normal group at 8 weeks after high fat diet. However, compared with the model group, the levels of serum AngⅡ, TNF-α, IL-6 and PAI-1 in the TCM group were significantly decreased after the intervention of Chinese herbs for clearing the liver and nourishing the kidney. The difference between IL-6 and normal group was not significant P> 0.05). The level of TNF-α was significantly lower than that of the normal group, while PAI-1 was significantly higher than that of the normal group (P <0.05). Conclusion Qingganzishen Chinese medicine can inhibit the expression of inflammatory cytokines in spontaneously hypertensive rats fed high-fat diet, which may be one of the mechanisms for its treatment of hypertension complicated with metabolic disorders.