用荧光光谱法研究了水溶液中抗癌药物丝裂霉素C、阿霉素与过氧化氢酶分子间的结合反应。结果表明 :药物对过氧化氢酶的内源荧光有较强的猝灭作用 ,形成复合物所产生的静态猝灭是引起过氧化氢酶荧光猝灭的主要原因。进一步依据荧光猝灭结果确定了药物 酶复合物的形成常数 The binding reaction of mitomycin C, adriamycin and catalase in aqueous solution was studied by fluorescence spectroscopy. The results show that the drug has a strong quenching effect on the endogenous fluorescence of catalase, and the static quenching caused by the complex formation is the main reason for the fluorescence quenching of catalase. Based on the fluorescence quenching results, the formation constants of the enzyme complexes were further determined