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目的和方法 :采用原位杂交技术 ,观察遗传性听源性癫痫易感大鼠海马内CCKmRNA表达的改变及海马CA3区注射CCK 8及其受体阻断剂对大鼠癫痫发作的影响。结果 :①癫痫发作大鼠海马内CCKmRNA表达明显增强(P <0 .0 5 0 .0 1 ) ;但癫痫反复发作的大鼠海马内CCKmRNA表达较癫痫发作一次大鼠明显减少 (P <0 .0 5 0 .0 1 ) ;②海马CA3 区注射CCK 8或L 36 4+CCK 8,大鼠癫痫发作明显减轻 (P <0 .0 5 ) ;海马CA3 区注射L 36 5后 ,CCK 8压抑癫痫发作的作用消失 (P <0 .0 1 )。结论 :CCK 8参与癫痫发作过程 ,且具有压抑癫痫发作作用 ,该作用可能是通过CCK B受体而实现的。
PURPOSE AND METHODS: The changes of CCK mRNA expression in the hippocampus of genetically-susceptible epileptic seizures and the effects of CCK 8 and its receptor blockers injected into hippocampal CA3 area on the seizures in rats were observed by in situ hybridization. Results: ① The expression of CCK mRNA in the hippocampus of seizure rats was significantly increased (P <0.05). However, the expression of CCK mRNA in the hippocampus of rats with recurrent seizures was significantly lower than that of the rats with seizure (P <0.05). (P <0.05). ②The levels of CCK 8 or L 36 4 + CCK 8 in the hippocampal CA3 area were significantly reduced (P0.05) The role of seizures disappeared (P <0.01). Conclusion: CCK 8 is involved in the process of seizure and has the effect of suppressing seizures. This effect may be achieved through the CCK B receptor.