微生物的生命活动往往会引起含水层堵塞，这一问题直接影响着人工微生物脱氮技术的推广和应用，为了寻求妥善的解决办法，笔者进行了大量的实验研究工作。本文就微生物堵塞问题的研究现状，堵塞机理以及含水层渗透系数随时间变化规律等予以阐述，以对今后的大面积推广提供一些参考。 Microbial life activities tend to cause aquifer clogging. This issue has a direct impact on the promotion and application of artificial microbial denitrification technology. In order to find a proper solution, the author conducted a large amount of experimental research. In this paper, the research status of microbial plugging, the mechanism of plugging and the law of permeability of aquifer over time are expounded in order to provide some references for large-scale popularization in the future.