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植物名称:白首乌(Cynanchum bungei)材料类别:带节茎段。无菌条件下将茎浸入70％酒精半分钟,再经0.1％升汞溶液消毒12分钟,然后用无菌水冲洗数次,切取0.5cm长带节的茎段,按其极性方向插入培养基中。培养条件:MS为基本培养基,蔗糖3％,琼脂0.6％,pH5.8。芽增殖培养基附加NAA0.05ppm
Plant Name: Cynanchum bungei Material Category: With stem segments. Stems were soaked in 70% alcohol for half a minute under aseptic conditions, sterilized by 0.1% mercuric chloride solution for 12 minutes, and then rinsed several times with sterile water. The stem segments of 0.5 cm long band were cut and inserted into the culture in the direction of their polarity Base. Culture conditions: MS as the basic medium, sucrose 3%, agar 0.6%, pH5.8. Bud proliferation medium added NAA 0.05ppm