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测试和筛选出14条多态性较高的省沽油ISSR有效引物,并对安徽省野生省沽油的遗传多样性进行初步调查分析。共扩增出143个多态性位点,居群水平平均有效等位基因数ne为1.271,期望杂合度He为0.165,Shannon多态性信息指数I为0.257,物种水平遗传多样性参数分别为ne=1.341,He=0.227,I=0.370;居群间遗传分化系数为Gst=0.271。初步揭示了野生省沽油的遗传多样性,同时表明ISSR标记对于研究省沽油遗传多样性的适用性和可靠性,研究结果将为进一步深入研究省沽油多居群的遗传多样性和资源保护提供帮助。
A total of 14 valid ISSR-efficient primers were tested and screened, and the genetic diversity of wild oil in Anhui province was investigated. A total of 143 polymorphic loci were amplified. The average effective number of alleles per population ne was 1.271, the expected heterozygosity He was 0.165, Shannon’s polymorphism information index I was 0.257, and the genetic diversity at species level was ne = 1.341, He = 0.227, I = 0.370; The coefficient of genetic differentiation among populations was Gst = 0.271. Preliminary revealed the genetic diversity of wild provincial oilseed oil, at the same time that the ISSR markers for the study of the applicability and reliability of the genetic diversity of the province, the results will further study the genetic diversity and resources Protection provides help.