论文部分内容阅读
为分离鉴定发生在福建省平和县的琯溪蜜柚黑斑病的病原菌,从果园中采集具有典型症状的病果,分离获得一种形态特征和rDNA-ITS序列与柑桔黑斑病菌不同的病菌分离物,并鉴定为柑桔叶点霉亚洲种。使用病菌分离物的无性世代的分生孢子及菌饼培养物,并以柑桔黑斑病菌作对照,接种柚果测定致病性。结果表明,病菌分离物回接产生与果园中病果相同的症状,并从接种发病的果实中重新分离到该病菌。因此,研究通过柯赫氏法则证实了柑桔叶点霉亚洲种是引起琯溪蜜柚黑斑病的病原菌。而对照菌株柑桔叶点霉也可以侵染琯溪蜜柚果实产生相同症状,有可能共同存在于琯溪蜜柚果园中为害。
In order to isolate and identify the pathogen of black spot disease in Guanxi honey pomelo in Pinghe County of Fujian Province, the diseased fruit with typical symptoms was collected from the orchard, and a morphological feature and rDNA-ITS sequence was isolated from the black spot pathogen The germ isolate was identified as an asian species of Phyttrium citrus. The asexual conidia and cocci cultures of the bacterial isolates were used, and the black spot pathogen was used as a control to inoculate the pomelo fruit to determine the pathogenicity. The results showed that the pathogen isolate returned to produce the same symptoms as the fruit in the orchard and the bacteria was re-isolated from the diseased fruit. Therefore, the study confirmed by Koch’s law that citrus leaf mold Asian species is caused by the black spot Caesar pomelo pathogen. The control strains of citrus frotilla can also invade Guanxi pomelo fruit produce the same symptoms, there may be common in Guanxi pomelo orchard damage.