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为查明鼻的防御机制,作者们研究了鼻局部抗体的产生、性质和功能。用无特异病原体的Wistar种大鼠(体重200～250克),5只作人工鼻阻塞,5只不阻塞,均放入玻璃瓶,用支气管败血性博代杆菌浸出液(5mg蛋白南)经雾化器喷入瓶内。首次暴露12小时,隔14天以同法再暴露一次,再隔10天,用被动血凝反应法测定受试鼠的血清和洗鼻液(1ml磷酸缓冲液经气管注入鼻咽腔经鼻流出)中抗支气管败血性博代杆菌的抗体。并用间接荧光抗体法测定洗鼻液中此种杆菌的特异性IgA抗体。第二步用20只鼠,10只作鼻阻塞,10只不阻塞,在作第二次暴露之后养10天,再放入瓶内,雾化吸入活的细菌细胞,细胞浓度调节到10~3/2.5ml磷酸缓冲液。另外,用10只鼠先作腹膜内注射细菌浸出液(0.02mg蛋白质),3周之后同
To pinpoint nasal defense mechanisms, the authors studied the production, properties, and function of nasal antibodies. Wistar rats without specific pathogen (weighing 200-250 g) were used. Five artificial obstructed nasal obstructions and five non-obstructed rats were placed in glass bottles. The mice were challenged with M. bronchiseptica leaching solution (5 mg protein) through fog Sprayer into the bottle. The first exposure 12 hours, 14 days in the same law and then exposed once again, and then separated by 10 days, with passive hemagglutination reaction test rat serum and nasal wash (1ml phosphate buffer transnasally nasopharyngeal outflow ) In anti-bronchiseptic bacteriophage antibodies. The specific IgA antibodies against this bacillus in the nasal wash were measured by indirect fluorescent antibody assay. The second step with 20 rats, 10 for nasal obstruction, 10 were not blocked, after the second exposure to raise 10 days, and then into the bottle, inhalation of live bacterial cells, the cell concentration adjusted to 10 ~ 3 / 2.5ml phosphate buffer. In addition, 10 mice were used for intraperitoneal injection of bacterial leachate (0.02 mg of protein), and three weeks later