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研究了盐酸奎宁(Quinine dihydrochloride,简称Quinine)与全氟辛烷磺酸(perfluorooctane sulfonate,简称PFOS)相互作用的共振光散射(resonance light scattering,RLS)光谱,并建立了PFOS的共振光散射分析方法.在pH值为2.87的Britton-Robinson(BR)缓冲溶液中,全氟辛烷磺酸根阴离子与质子化的盐酸奎宁通过静电引力和疏水作用形成2:1的离子缔合物,引起共振光散射强度(IRLS)显著增强,最大散射波长位于283nm处,增强的散射信号强度与PFOS浓度在0.10～50.0μmol/L范围内呈线性关系,据此建立了测定PFOS的散射分析方法,检测限为9.88nmol/L.讨论了体系的最佳反应条件及外来物质的干扰,同时研究了体系的吸收光谱及荧光光谱,并探讨了反应机理.本方法用于水样及人体血清样品中PFOS的测定,RSD≤4.2%.
The resonance light scattering (RLS) spectrum of the interaction between Quinine dihydrochloride (Quinine) and perfluorooctane sulfonate (PFOS) was studied, and the resonance light scattering analysis of PFOS Methods The perfluorooctane sulfonate anion and protonated quinine hydrochloride formed a 2: 1 ion association by electrostatic attraction and hydrophobic interaction in a Britton-Robinson (BR) buffer solution at a pH of 2.87, causing resonance The light scattering intensity (IRLS) was significantly enhanced, the maximum scattering wavelength was at 283nm, and the enhanced scattering signal intensity was linear with the concentration of PFOS in the range of 0.10-50.0μmol / L. Based on this, a scattering analysis method for the determination of PFOS was established. The detection limit Was 9.88nmol / L. The optimal conditions of the system and the interference of foreign substances were discussed, the absorption and fluorescence spectra of the system were also studied, and the reaction mechanism was discussed.The method was applied to the determination of PFOS in water and human serum samples Determination, RSD ≤ 4.2%.