论文部分内容阅读
目的探讨麻黄汤不同给药时间抗小鼠心肌炎病毒的作用,明确该药的最佳给药时机。方法选择8周龄C3H鼠116只,取29只为正常空白组,其余腹腔注射0.01%的心肌炎病毒(EMBV)0.1 mL后随机分为3组(n=29):模型组0~4 d给予生理盐水0.2 mL灌胃;麻黄汤1组在0~4 d灌服麻黄汤0.2 mL(500 mg/kg.d);麻黄汤2组在2~6 d灌服麻黄汤0.2 mL。各组随机取11只观察生存时间,其余在2、4、6、14 d分别观察胸腺指数、心脏指数、肺脏指数、脾脏指数,心脏常规HE染色后观察心肌坏死情况。结果模型组和麻黄汤1组的7 d生存率均为18.18%,麻黄汤2组为63.64%,模型组和麻黄汤1组的14 d生存率为0%,麻黄汤2组为36.36%(P<0.05)。模型组和麻黄汤1组的体重在6 d明显低于麻黄汤2组,模型组心脏重量在4 d明显上升,其心脏指数与麻黄汤1组、麻黄汤2组相比有显著性差异(P<0.05)。至6 d,麻黄汤2组的心脏重量和心脏指数明显低于模型组(P<0.01),麻黄汤2组的心脏指数在4 d和6 d均低于麻黄汤1组(P<0.01),麻黄汤1组、麻黄汤2组肺脏指数在4、6 d低于模型组,麻黄汤2组肺脏指数在4 d低于麻黄汤1组,麻黄汤1组的脾脏指数在2 d高于模型组(P<0.01),麻黄汤2组的脾脏指数在6 d高于模型组(P<0.05)。心脏病理也显示病毒感染后2 d给麻黄汤心肌坏死明显减轻。结论心肌炎病毒感染后2 d给麻黄汤疗效优于同时给药;提示感染后2 d给麻黄汤,小鼠胸腺、脾脏及心脏能产生适度的免疫反应。
Objective To investigate the anti-rat myocarditis virus effect of different doses of Mahuang Decoction, and to determine the optimal timing of drug administration. Methods One hundred and sixteen-week-old C3H mice were selected and 29 of them were taken as normal blank group. The rest of the rats were injected intraperitoneally with 0.01% of myocarditis virus (EMBV) 0.1 mL and randomly divided into 3 groups (n=29): 0 to 4 days in the model group. 0.2 mL of normal saline was given to the stomach; Mahuang Decoction 1 group was fed 0.2 mL (500 mg/kg.d) of Mahuang Decoction on 0-4 days; Mahuang Decoction 2 group was given 0.2 mL of Mahuang Decoction on 2-6 days. All 11 groups were randomly selected to observe the survival time, and the rest were observed on 2, 4, 6 and 14 days respectively. The thymus index, heart index, lung index, and spleen index were observed. After cardiac HE staining, the myocardial necrosis was observed. Results The 7-day survival rate was 18.18% in the model group and the Mahuang Decoction 1 group, 63.64% in the Mahuang Decoction 2 group, 0% in the model group and Mahuang Decoction 1 group, and 36.36% in the Mahuang Decoction 2 group ( P<0.05). The body weight of the model group and the Mahuang Decoction 1 group was significantly lower than that of the Mahuang Decoction 2 group. The cardiac weight of the model group increased significantly on the 4th day, and the cardiac index was significantly different from the Mahuang Decoction 1 and Mahuang Decoction 2 groups. P<0.05). At 6 days, the heart weight and heart index of Mahuang Decoction 2 group were significantly lower than that of the model group (P<0.01), and the cardiac index of Mahuang Decoction 2 group was lower than Mahuang Decoction 1 group on the 4th and 6th days (P<0.01). The lung index of Mahuang Decoction 1 group and Mahuang Decoction 2 group were lower than the model group on the 4th and 6th days. The lung index of Mahuang Decoction 2 group was lower than Mahuang Decoction 1 group on the 4th day, and the spleen index of Mahuang Decoction 1 group was higher on the 2nd day. In the model group (P<0.01), the spleen index of the Mahuang Decoction 2 group was higher than that of the model group on the 6th day (P<0.05). Cardiac pathology also showed that myocardial necrosis was significantly reduced in Mahuang Decoction 2 days after viral infection. Conclusion Mahuang Decoction is more effective than Simultaneous Administration at 2 days after myocarditis virus infection. It suggests that Mahuang Decoction and mouse thymus, spleen and heart can produce moderate immune responses at 2 days after infection.