论文部分内容阅读
目的 研究葡糖酰基鞘氨醇合酶抑制剂苯基棕榈酰胺吗啡丙醇 (PPMP)对耐阿霉素卵巢癌细胞亚株SKOV3 AdrR的多药耐药基因mdr1及P 糖蛋白的调节。方法 应用细胞培养技术对SKOV3 AdrR细胞进行PPMP处理 ,采用逆转录 聚合酶链反应技术分析mdr1mRNA的表达 ,流式细胞仪检测细胞内罗丹明的荧光强度。结果 浓度为 5、15 μmol L的PPMP可部分抑制SKOV3 AdrR细胞mdr1mRNA的表达 ,2 5 μmol L的PPMP可完全抑制SKOV3 AdrR细胞mdr1mRNA的表达 ,调节作用呈浓度依赖性。PPMP可增加SKOV3 AdrR细胞内罗丹明的荧光强度 ,随着PPMP浓度 (分别为 5、15、2 5μmol L)的增加 ,细胞内罗丹明的荧光强度逐渐增高 (分别为 3 0 1 2 2、3 89 98、4 2 6 0 8)。 结论 PPMP可逆转SKOV3 AdrR的多药耐药性。
Objective To investigate the regulation of the multidrug resistance gene mdr1 and P glycoprotein of the adriamycin-resistant ovarian cancer subpopulation SKOV3 AdrR by the glucosyl-sphingosine synthase inhibitor phenylpalmitoyl-morphine-propanol (PPMP). Methods SKOV3 AdrR cells were treated with PPMP by cell culture technique. The expression of mdr1 mRNA was analyzed by reverse transcription polymerase chain reaction. The fluorescence intensity of rhodamine in the cells was detected by flow cytometry. Results The concentration of mdr1 mRNA in SKOV3 AdrR cells was partially inhibited by PPMP at concentrations of 5 and 15 μmol L. The expression of mdr1 mRNA in SKOV3 AdrR cells was completely inhibited by 25 μmol L of PPMP in a concentration-dependent manner. PPMP increased the fluorescence intensity of rhodamine in SKOV3 AdrR cells. With the increase of PPMP concentrations (5, 15, 25 μmol L), the fluorescence intensity of intracellular rhodamine increased gradually (3012, 3, respectively). 89 98, 4 2 6 0 8). Conclusion PPMP can reverse the multidrug resistance of SKOV3 AdrR.