目的 :探讨NDRG2对肝癌细胞HepG2凋亡的诱导作用。方法 :用RT PCR获取NDRG2基因。测序判断正确后 ,将其克隆入含绿色荧光蛋白 (GFP)基因的真核表达载体pIRES2 EGFP中 ,并转染NDRG2表达阴性的HepG2细胞。用光镜观察转染细胞的形态变化 ;荧光显微镜观察NDRG2蛋白的定位 ;流式细胞仪分析细胞周期的改变 ;透射电镜进一步观察细胞超微结构的改变。结果 :获得了NDRG2基因 ,成功地构建了 pIRES2 EGFP NDRG2真核表达载体。转染HepG2细胞后 ,细胞生长受抑 ,结构破坏并死亡。荧光显微镜观察到NDRG2在胞质中表达 ,流式细胞仪检测出现G1期阻滞和凋亡峰 ,透射电镜观察到典型的细胞凋亡特征。结论 :NDRG2基因具有诱导HepG2细胞凋亡的作用 ,其机制有待进一步研究 Objective: To investigate the effect of NDRG2 on the apoptosis of HepG2 cells. Methods: The NDRG2 gene was obtained by RT PCR. After sequencing, the recombinant plasmid was cloned into eukaryotic expression vector pIRES2 EGFP containing green fluorescent protein (GFP) gene and transfected into HepG2 cells with negative expression of NDRG2. The morphological changes of transfected cells were observed with light microscope. The localization of NDRG2 protein was observed by fluorescence microscopy. The cell cycle was analyzed by flow cytometry. The ultrastructural changes were observed by transmission electron microscopy. Results: The NDRG2 gene was obtained and the eukaryotic expression vector pIRES2 EGFP NDRG2 was successfully constructed. After transfection HepG2 cells, cell growth was inhibited, the structure destroyed and died. Fluorescence microscopy showed that NDRG2 was expressed in the cytoplasm, G1 arrest and apoptosis peak were detected by flow cytometry, and typical apoptotic characteristics were observed by transmission electron microscopy. Conclusion: The NDRG2 gene has the effect of inducing HepG2 cell apoptosis. The mechanism needs to be further studied