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目的 探讨树突状细胞 -结肠癌细胞融合在活化T细胞主要组织相容性复合体 (MHC)限制性反应中的作用。方法 使用 5 0 %聚乙二醇将人外周血树突状细胞与结肠癌细胞SW4 80融合 ,用其致敏T淋巴细胞 ,观察T细胞的活化情况。结果 融合细胞与T淋巴细胞混合后 ,CD4 + Th1细胞和CD8+ Tc1细胞的活化率分别为 5 7 4 5 %和 77 86 % ,而对照组无明显活化 ;T细胞的增殖明显高于对照组 (P <0 0 1) ;T细胞分泌的IFN γ明显高于对照组 (P <0 0 1)。结论 树突状 -肿瘤细胞融合策略能有效地活化抗肿瘤MHC Ⅰ类反应和Ⅱ类反应 ,促进T细胞增殖。
Objective To investigate the role of dendritic cell-colon cancer cell fusion in the regulatory T cell major histocompatibility complex (MHC) response. Methods Human peripheral blood mononuclear cells were fused with human colon cancer cell SW480 using 50% polyethylene glycol (PEG) to sensitize T lymphocytes and observe the activation of T cells. Results The percentages of activation of CD4 + Th1 cells and CD8 + Tc1 cells were 57.45% and 77.86%, respectively, but not in the control group. The proliferation of T cells was significantly higher than that of the control group P <0.01); IFN-γ secreted by T cells was significantly higher than that of the control group (P <0.01). Conclusion Dendritic-tumor cell fusion strategy can effectively activate anti-tumor MHC class I and class II responses and promote T cell proliferation.