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【据《Virology》2014年6月报道】题:用于培养1-6型HCV颗粒的免血清高滴度培养体系的生产和特征(作者Mathiesen CK等)最近制造HCV颗粒(HCVcc)的细胞培养系统被研发出来。构建无血清培养环境可能更利于无活性全病毒疫苗的发展。丹麦哥本哈根卫生和医学科学学院Mathiesen CK等描述了通过培养于腺病毒的Huh7.5细胞表达介质获得基因型1-6型的无血清HCVcc(sf-HCVcc)。与HCVcc相比,sf-HCVcc显示出0.6~2.1 log10倍(4.7~6.2 log10Focus Forming Units/ml)的感染性滴度的增加,可能与sf-HCVcc的释放增加和特异性感染力相关。与HCVcc相反,sf-HCVcc在密度梯度谱中只有一个同源性高峰。sf-HCVcc的进入依赖于HCV共同受
Virology, June 2014 Report Title: Production and Characterization of a Serum-free, High-titer Culture System for Type 1-6 HCV Particles by Mathiesen CK, et al. Recently, cell cultures of HCV particles (HCVcc) The system was developed. Building a serum-free culture environment may be more conducive to the development of an inactive whole virus vaccine. Mathiesen CK et al., Copenhagen School of Hygiene and Medical Sciences, Denmark, described that serum-free HCVcc (sf-HCVcc) of genotype 1-6 was obtained by culturing Huh7.5 cell expressing adenovirus. Compared to HCVcc, sf-HCVcc showed an increase of infectious titer of 0.6 to 2.1 log10 fold (4.7 to 6.2 log10 Focus Forming Units / ml), possibly related to the increased release of sf-HCVcc and the specific infectivity. In contrast to HCVcc, sf-HCVcc has only one homology peak in the density gradient profile. The entry of sf-HCVcc depends on HCV concurrence