核酸适配体(Aptamer)是一类单链短DNA或RNA片段,具有一定的空间结构,对特定靶分子具有亲和力。本研究采用指数富集配体系统进化技术(SELEX),首次筛选出针对鲤春病毒血症病毒(SVCV)的核酸适配体A2和A15,经过荧光PCR、凝胶阻滞和病毒中和实验,证明了其对SVCV具有结合力和抑制作用,在该病的检测和防治方面具有良好的前景。 Aptamer is a kind of short single-stranded DNA or RNA fragment with a certain spatial structure and affinity for a specific target molecule. In this study, exponential enrichment ligand system evolutionary technique (SELEX) was used to screen the aptamers A2 and A15, which are against SVCV, for the first time. After fluorescence PCR, gel retardation and virus neutralization , Which proved that it has the binding force and inhibitory effect on SVCV and has good prospects in the detection and prevention of the disease.