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将4种微生物基因组DNA腹腔注射罗非鱼(Oreochromis niloticus),48 h后腹腔注射海豚链球菌,在攻毒后24和48 h采血,通过测定血清溶菌酶(LSZ)、超氧化物歧化酶(SOD)和碱性磷酸酶(AKP)活性的变化,以及血液中病原菌感染量和死亡率,探讨注射4种微生物DNA对罗非鱼抗海豚链球菌感染的作用。结果显示,注射大肠杆菌DNA的实验组,LSZ、AKP和SOD均无明显变化(P>0.05),表现为先略低于对照组后升至正常水平;而注射鮰爱德华氏菌DNA、毕赤酵母DNA和生理盐水的实验鱼,LSZ和AKP表现出明显变化(P<0.05),LSZ变化趋势为先增加后降低,而AKP变化趋势则不同。注射感染后除对照组外,其他处理组均有不同程度的细菌感染,其中24 h大肠杆菌组感染量显著低于生理盐水组(P<0.05),而48 h各试验组的细菌感染量均显著低于生理盐水组(P<0.05),尤其以大肠杆菌组和嗜水气单胞菌组最低,且其死亡率也最低,表明注射一定量的大肠杆菌基因DNA和嗜水气单胞菌基因DNA能增强罗非鱼抗海豚链球菌感染能力。
Four kinds of microbial genomic DNA were injected intraperitoneally into Oreochromis niloticus. After 48 h, S. iniae was inoculated intraperitoneally and blood was collected 24 and 48 h after challenge. The levels of serum lysozyme (LSZ), superoxide dismutase (SOD) and alkaline phosphatase (AKP), and the infection rate and death rate of pathogenic bacteria in the blood to investigate the effect of injecting 4 kinds of microbial DNA on S. tilapia anti-Infection. The results showed that there was no significant change in the experimental group injected with E.coli DNA (LSZ, AKP and SOD) (P> 0.05), the performance was slightly lower than the control group before moving to normal levels; while the injection of DNA of E. bovis, The experimental fish, LSZ and AKP of yeast DNA and saline showed significant changes (P <0.05). The trend of LSZ increased first and then decreased, while the change trend of AKP was different. Infection with infection except for the control group, the other treatment groups have varying degrees of bacterial infection, of which 24 h infection of E. coli group was significantly lower than the saline group (P <0.05), and 48 h in each test group bacterial infection were Significantly lower than the saline group (P <0.05), especially in the Escherichia coli group and Aeromonas hydrophila group the lowest, and the lowest mortality, indicating that a certain amount of injection of E. coli DNA and Aeromonas hydrophila Gene DNA enhances the ability of tilapia to infect S. syphilis.