目的研究α-细辛醚对大鼠星型胶质细胞活化的影响。方法诱发大鼠癫痫持续状态(SE)1 h,用胶质纤维酸性蛋白(GFAP)免疫组化染色观察正常组、模型组和实验组的大鼠海马区的星型胶质细胞活化情况;原代大鼠海马星型胶质细胞传代后,分为正常组、脂多糖诱导活化模型组和实验组;用CCK-8试剂盒检测各组细胞的增殖情况,用Western-blotting法检测各组GFAP表达。结果模型组在SE后第1天的海马星型胶质细胞活化最明显,在第28天恢复至正常水平;实验组的星型胶质细胞活化水平在第1天、第3天和第7天均低于模型组(P<0.05)。高、低2个剂量(50,100μg·m L-1)α-细辛醚对体外脂多糖诱导的星型胶质细胞的增殖有明显抑制作用(P<0.05);高浓度实验组对脂多糖诱导星型胶质细胞的GFAP表达增多有明显抑制作用(P<0.05)。结论α-细辛醚对大鼠海马星型胶质细胞的活化有抑制作用。 Objective To study the effects of α-asarone on activation of astrocytes in rats. Methods The rats were induced by epilepsy (SE) for 1 h, and the activation of astrocytes in hippocampus of rats in normal group, model group and experimental group were observed by immunohistochemical staining with glial fibrillary acidic protein (GFAP) The rat hippocampal astrocytes were passaged and divided into normal group and lipopolysaccharide-induced activation model group and experimental group. The proliferation of each group was detected by CCK-8 kit, and the expression of GFAP in each group was detected by Western-blotting expression. Results The activation of hippocampal astrocytes in the model group was most obvious on the 1st day after SE, and returned to the normal level on the 28th day. The activation level of astrocytes in the experimental group was significantly higher than that on the 1st, 3rd and 7th day The average daily mean was lower than the model group (P <0.05). High and low dose (50, 100μg · m L-1) α-asarone could significantly inhibit the proliferation of astrocytes induced by lipopolysaccharide in vitro (P <0.05) Induced GFAP expression in astrocytes significantly inhibited (P <0.05). Conclusion α-asarone can inhibit the activation of hippocampal astrocytes in rats.