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作者取波多黎各株曼氏血吸虫尾蚴,分别用机械法获得童虫、尾静脉注射法获得肺内的虫体或感染法获得成虫。从感染8周小鼠肝脏分离虫卵,经孵化收集毛蚴。将童虫经脱胆酸盐提取后的产物,免疫BALB/c鼠,取脾脏,进行细胞融合和克隆化培养,上清液用于实验。童虫培养于不含蛋氨酸的培养液中,加入[~(35)S]蛋氨酸于37℃、7％CO_2培养16小时,然后用脱胆酸盐处理。经ELISA和间接免疫荧光试验分析鉴定。将所有毛蚴、童虫、肺内的虫体和成虫抗原用去垢剂处理,以~(135)I
The authors took Schistosoma mansoni cercariae of Puerto Rico and acquired the parasites by the mechanical method and the adult worms by the injection of the tail vein in the lungs or by the infection method. Egg eggs were isolated from the liver of mice infected for 8 weeks and collected for miracidia. The Schistosoma japonicum was immunized with the product of the extraction of the cholic acid salt, BALB / c mice were immunized, spleens were harvested, cell fusion and clonal culture were performed, and the supernatant was used in the experiment. Schistosoma japonicum was cultured in methionine-free medium and incubated with [~ (35) S] methionine for 16 hours at 37 ° C and 7% CO 2, followed by treatment with an alkylate. By ELISA and indirect immunofluorescence assay analysis and identification. All miracidia, pest, lung worms and adult antigens with detergents to ~ (135) I