【摘 要】
：
目的:建立测定痔炎消颗粒中芦丁含量的方法。方法:采用高效液相色谱法。色谱柱为Merck Lichrospher C18柱(250 mm×4.6 mm,5μm);流动相为甲醇-1%冰乙酸(32∶68);流速1 mL.mi
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目的:建立测定痔炎消颗粒中芦丁含量的方法。方法:采用高效液相色谱法。色谱柱为Merck Lichrospher C18柱(250 mm×4.6 mm,5μm);流动相为甲醇-1%冰乙酸(32∶68);流速1 mL.min-1;检测波长为257 nm。结果:芦丁回归方程为Y=1 865.5X+101.61,r=0.999 5;线性范围为0.299~8.970μg(n=6,RSD=0.91%),平均回收率为99.54%,RSD为0.91%。结论:所建方法简便可行,快速,可用于痔炎消颗粒的质量控制。
Objective: To establish a method for determination of rutin in Qu Yan Xiao granules. Method: High performance liquid chromatography. The column was a Merck Lichrospher C18 column (250 mm×4.6 mm, 5 μm); the mobile phase was methanol-1% glacial acetic acid (32:68); the flow rate was 1 mL.min-1; the detection wavelength was 257 nm. Results: The regression equation of rutin was Y=1 865.5X+101.61, r=0.999 5. The linear range was 0.299 to 8.970 μg (n=6, RSD=0.91%). The average recovery was 99.54% and the RSD was 0.91%. Conclusion: The method is simple, feasible and rapid. It can be used to control the quality of Yanyanxiao granules.
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