论文部分内容阅读
目的观察幽门螺杆菌（Ｈｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉ，Ｈｐ）在常温保存液中的存活能力及影响因素，通过实际菌株传递实验，为我国Ｈｐ菌株库建立中各地Ｈｐ的收集和供应，提供切实可行的菌株传递方案．方法取新鲜培养３ｄ的Ｈｐ３株（ＮＣＴＣ１１６３７，ＮＣＴＣ１１６３９和ＣＡＰＭＮ６２），用Ｈｐ保存液（含１０ｇ／Ｌ蔗糖，５００ｍＬ／Ｌ小牛血清）作成１０１３ｃｆｕ／Ｌ浓度的菌悬液，分别以满管和半管形式装于１５ｍＬ灭菌小管中，经密封后放于３７℃恒温箱中，于１２，２４，３６，４８，６０，７２，９６，１２０，１４４，和１６８ｈ，按每种各取出一管，接种双份平皿（１００ｍＬ／Ｌ羊血布氏琼脂）后，于微需氧环境培养３ｄ后观察细菌生长情况．另外，将一满管１０１３ｃｆｕ／Ｌ浓度的Ｈｐ用特快专递（ＥＭＳ）自北京传送到上海，收到后马上进行接种，并连续观察传递管中菌株在不同时间内的存活情况．结果在３７℃保存液中Ｈｐ至少可存活７２ｈ，保存管中通过加满保存液减少空气残留，能明显延长Ｈｐ的存活时间；在气温２０℃～３４℃条件下，经ＥＭＳ实际传递的菌株存活良好，且在传递到达后可继续存活至５５ｈ．结论使用含１０％蔗糖的５０％小牛血清细菌传送保护液，在?
Objective To observe the viability and influencing factors of helicobacter pylori (Helicobacter pylori) in liquid at room temperature, and to provide a feasible solution for Hp strain collection and supply in China by establishing a practical strain transfer experiment. Methods Hp3 strain (NCTC11637, NCTC11639 and CAPMN62) cultured freshly for 3 days was prepared. The bacterial suspension with a concentration of 1013 cfu / L was prepared with Hp preservation solution (containing 10 g / L sucrose and 500 mL / L fetal bovine serum) The tube was placed in a 1 .5mL sterile tube, sealed and placed in a 37 ° C incubator at 12, 24, 36, 48, 60, 72, 96, 120, 144, and 168h, One tube was inoculated with duplicate plates (100 mL / L sheep blood serum Brinell agar) and the growth of bacteria was observed after 3 days culture in microaerobic environment. In addition, a full tube of Hp at a concentration of 1013 cfu / L was sent by EMS from Beijing to Shanghai. Immediately after inoculation, Hp was continuously observed for the survival of the strains in the delivery tube at different times. The results showed that Hp could survive for at least 72h in the preservation solution at 37 ℃. The preservation of the Hp could prolong the survival time of the Hp by filling the preservation solution in the storage tube. The strain actually delivered by EMS survived at the temperature of 20 ℃ ~ 34 ℃ Good, and can continue to survive in the delivery arrived 55h. Conclusions Using 50% bovine serum-containing 10% sucrose-containing bacterial protection solution,