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报道了我国北京和广州地区散发性戊型肝炎病毒核苷酸和氨基酸序列的测定结果。采集北京、广州两地1993年散发的戊型肝炎(HE)病人急性期血清,取抗-HEV阳性的血清抽提病毒RNA,用HEVET1.1保守区中的特异引物反转录合成cDNA,再用两对引物进行套式聚合酶链反应(RT-PCR),然后将阳性的PCR产物直接进行序列分析,共获得3份HEV序列,并推测其氨基酸序列。将这3份HEV(210bp)序列与国内外已知的HEV相应序列(4545~4754)进行比较,结果从北京一名病人(B-9)中检出与缅甸株一致的序列,核苷酸和氨基酸序列的同源性为100%;从广州两名病人(G-9和G-20)中检出的序列均不同于国内外已知HEV的序列,其核苷酸的同源性为74.3%~67.1%;氨基酸的差异性为14.3%~22.9%。经分析可能为一种新的HEV基因型,这将为HE的防治和诊断提供科学依据。
The nucleotide and amino acid sequences of sporadic HEV in Beijing and Guangzhou of China were reported. Acute hepatitis E (HE) sera from Beijing and Guangzhou were collected from Beijing and Guangzhou in 1993 to collect anti-HEV-positive serum RNA for RNA extraction. The cDNA was reverse transcribed using specific primers in the conserved region of HEVET1.1 Two sets of primers were used for nested-PCR (RT-PCR), and then the positive PCR products were directly sequenced. Three HEV sequences were obtained and their amino acid sequences were deduced. The three HEV (210bp) sequences were compared with the corresponding known HEV sequences (4545 ~ 4754) at home and abroad. The sequence was identical to the Myanmar strain in one patient (B-9) in Beijing. The nucleotide And 100% homology with the amino acid sequence. The sequences from two patients (G-9 and G-20) in Guangzhou were different from the sequences of known HEV at home and abroad. The nucleotide homology was 74.3% ~ 67.1%; The amino acid difference was 14.3% ~ 22.9%. The analysis may be a new HEV genotype, which will provide a scientific basis for the prevention and diagnosis of HE.