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基于表面引发接枝聚合,设计与建立了一种新的分子表面印迹(MIP)方法.先使用偶联剂γ-氨丙基三甲氧基硅烷(AMPS)对微米级硅胶微粒进行表面改性,制得改性硅胶AMPS-SiO2;在酸性水溶液体系中,凭借强静电相互作用,阴离子单体对苯乙烯磺酸钠(PSSS)被结合在模板分子抗蚜威周围;改性硅胶AMPS-SiO2表面的氨基与溶液中的过硫酸盐构成氧化还原引发体系,在硅胶微粒表面产生自由基,引发结合在模板分子周围的SSS及交联剂甲基双丙烯酰胺(MBA)在硅胶微粒表面发生接枝交联聚合,从而实现了抗蚜威分子的表面印迹,制得了抗蚜威分子表面印迹材料MIP-PSSS/SiO2.以残杀威为对比物,采用静态与动态两种方法,考察研究了分子表面印迹材料MIP-PSSS/SiO2对抗蚜威的分子识别特性与结合性能.研究结果表明,印迹材料MIP-PSSS/SiO2对抗蚜威分子具有特异的识别选择性与优良的结合亲和性,相对于残杀威分子,MIP-PSSS/SiO2对抗蚜威分子的识别选择性系数为9.373,显示出高的分子识别选择性.
Based on the surface-initiated graft polymerization, a new molecular surface imprinting (MIP) method was designed and developed.Microspherical silica particles were surface modified with γ-aminopropyltrimethoxysilane (AMPS) (AMPS-SiO2). In acidic aqueous solution, the anionic monomer PSSS was bound around the template molecule of pirimicarb by strong electrostatic interaction. The surface of the modified silica gel AMPS-SiO2 Of amino and persulfate in the solution constitute the redox initiating system to generate free radicals on the surface of the silica gel particles to initiate the binding of the SSS and the crosslinking agent methyl bisacrylamide (MBA) bound around the template molecules to the surface of the silica gel particles Cross-linked polymerization, in order to achieve the surface of the pirimicarb molecular imprinting, obtained prickly heat molecular imprinting material MIP-PSSS / SiO2.With the propoxur as the contrast, using static and dynamic methods to study the molecular surface MIP-PSSS / SiO2 imprinted molecular identification characteristics and binding properties against pirimicarb.The results show that the imprinted material MIP-PSSS / SiO2 has specific recognition selectivity and excellent binding affinity with pirimicarb, as compared with Wei kill molecule, MIP-PSSS / SiO2 selectivity coefficient against pirimicarb recognition molecule is 9.373, exhibit high selective molecular recognition.