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目的探讨二十二碳六烯酸(DHA)对食管腺癌(EAC)和食管鳞癌(ESCC)细胞增殖调节过程中脂质过氧化反应(LP0)的影响。方法 DHA分别以100,50,1μmo1/L干预Eca-109细胞(ESCC)及0E-19细胞(EAC)24h,设溶质对照组(0.1%乙醇)和空白对照组。四唑单钠盐(CCK-8)法检测各组细胞增殖情况;WST—1法检测细胞内总超氧化物歧化酶(SOD)活性、TBA法检测总丙二醛(MDA)含量及硝酸还原酶法检测一氧化氮(N0)水平。结果 DHA引起0E-19浓度依赖性细胞增殖抑制(P<0.0 5),仅高浓度(100μmol/L)时抑制Eca—109曾殖(P<0.05);干预24h后,MDA含量和NO水平在Eca-109及OE-19各组细胞均出现DHA浓度依赖性上升(P<0.05);S0D活性在中低浓度DHA干预的Eca-109细胞无明显变化,但在高浓度组显著下降(P<0.05);OE-19细胞的SOD活性在中低浓度DHA干预后明显下调,而在高浓度组显著上升(P<0.05)。结论 DHA可稳定抑制EAC细胞而非ESCC细胞增殖。DHA干预后,无论是EAC还是ESCC均发生显著的LP0,其与抗氧化物质SOD表达之间的相对平衡可能是影响肿瘤细胞增殖的重要因素。
Objective To investigate the effects of docosahexaenoic acid (DHA) on lipid peroxidation (LPO) during the process of esophageal adenocarcinoma (EAC) and esophageal squamous cell carcinoma (ESCC) proliferation. Methods DHA were exposed to Eca-109 cells (ESCC) and 0E-19 cells (EAC) at 100, 50 and 1μmo1 / L for 24 hours respectively, and control group (0.1% ethanol) and blank control group were established. Cell viability was measured by CCK-8 assay. The activity of total superoxide dismutase (SOD) was assayed by WST-1 assay. The contents of total malondialdehyde (MDA) and nitric acid Enzymatic detection of nitric oxide (N0) levels. Results DHA induced inhibition of Eca-109 concentration-dependent cell proliferation in a concentration-dependent manner (P <0.05), and inhibition of Eca-109 expression at high concentrations (100 μmol / L) The concentration of DHA in Eca-109 and OE-19 groups increased in a concentration-dependent manner (P <0.05). There was no significant change in S0D activity in Eca-109 cells intervened by medium and low concentration of DHA, but significantly decreased in high concentration group (P < 0.05). The SOD activity of OE-19 cells was significantly down-regulated after low and medium DHA intervention, but significantly increased in high concentration group (P <0.05). Conclusion DHA can stably inhibit the proliferation of EAC cells but not ESCC cells. After DHA intervention, significant LPO occurred in both EAC and ESCC, and its relative balance with antioxidant SOD expression may be an important factor affecting tumor cell proliferation.